Imperial College London
Alternate

ProfessorJesusGil

Faculty of Medicine, Institute of Clinical Sciences

Professor of Cell Proliferation
 
 
 
//

Contact

 

+44 (0)20 3313 8263jesus.gil

 
 
//

Location

 

ICTEM room 230ICTEM buildingHammersmith Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Bagnati:2019:10.1172/jci.insight.125736,
author = {Bagnati, M and Moreno-Moral, A and Ko, J-H and Nicod, J and Harmston, N and Imprialou, M and Game, L and Gil, J and Petretto, E and Behmoaras, J},
doi = {10.1172/jci.insight.125736},
journal = {JCI insight},
title = {Systems-genetics identifies a macrophage cholesterol network associated with physiological wound healing},
url = {http://dx.doi.org/10.1172/jci.insight.125736},
volume = {4},
year = {2019}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Among other cells, macrophages regulate the inflammatory and reparative phases during wound healing but genetic determinants and detailed molecular pathways that modulate these processes are not fully elucidated. Here, we took advantage of normal variation in wound healing in 1,378 genetically outbred mice, and carried out macrophage RNA-sequencing profiling of mice with extreme wound healing phenotypes (i.e., slow and fast healers, n = 146 in total). The resulting macrophage coexpression networks were genetically mapped and led to the identification of a unique module under strong trans-acting genetic control by the Runx2 locus. This macrophage-mediated healing network was specifically enriched for cholesterol and fatty acid biosynthetic processes. Pharmacological blockage of fatty acid synthesis with cerulenin resulted in delayed wound healing in vivo, and increased macrophage infiltration in the wounded skin, suggesting the persistence of an unresolved inflammation. We show how naturally occurring sequence variation controls transcriptional networks in macrophages, which in turn regulate specific metabolic pathways that could be targeted in wound healing.
AU  - Bagnati,M
AU  - Moreno-Moral,A
AU  - Ko,J-H
AU  - Nicod,J
AU  - Harmston,N
AU  - Imprialou,M
AU  - Game,L
AU  - Gil,J
AU  - Petretto,E
AU  - Behmoaras,J
DO  - 10.1172/jci.insight.125736
PY  - 2019///
SN  - 2379-3708
TI  - Systems-genetics identifies a macrophage cholesterol network associated with physiological wound healing
T2  - JCI insight
UR  - http://dx.doi.org/10.1172/jci.insight.125736
UR  - http://hdl.handle.net/10044/1/66898
VL  - 4
ER  -
Download