Imperial College London
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Dr John S Tregoning

Faculty of Medicine, Department of Infectious Disease

Professor in Vaccine Immunology
 
 
 
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Contact

 

john.tregoning Website

 
 
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Location

 

456 (Shattock Group)Wright Fleming WingSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Mcdonald:2016:10.1128/mSystems.00051-16,
author = {Mcdonald, J and Kaforou, M and Clare, S and Hale, C and Ivanova, M and Huntley, D and Dorner, M and Wright, VJ and levin, M and Torres, FM and Herberg, J and Tregoning, JS},
doi = {10.1128/mSystems.00051-16},
journal = {mSystems},
title = {A Simple Screening Approach To Prioritize Genes for Functional Analysis Identifies a Role for Interferon Regulatory Factor 7 in the Control of Respiratory Syncytial Virus Disease},
url = {http://dx.doi.org/10.1128/mSystems.00051-16},
volume = {1},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Greater understanding of the functions of host gene products in response to infection is required. While many of these genes enable pathogen clearance, some enhance pathogen growth or contribute to disease symptoms. Many studies have profiled transcriptomic and proteomic responses to infection, generating large data sets, but selecting targets for further study is challenging. Here we propose a novel data-mining approach combining multiple heterogeneous data sets to prioritize genes for further study by using respiratory syncytial virus (RSV) infection as a model pathogen with a significant health care impact. The assumption was that the more frequently a gene is detected across multiple studies, the more important its role is. A literature search was performed to find data sets of genes and proteins that change after RSV infection. The data sets were standardized, collated into a single database, and then panned to determine which genes occurred in multiple data sets, generating a candidate gene list. This candidate gene list was validated by using both a clinical cohort and in vitro screening. We identified several genes that were frequently expressed following RSV infection with no assigned function in RSV control, including IFI27, IFIT3, IFI44L, GBP1, OAS3, IFI44, and IRF7. Drilling down into the function of these genes, we demonstrate a role in disease for the gene for interferon regulatory factor 7, which was highly ranked on the list, but not for IRF1, which was not. Thus, we have developed and validated an approach for collating published data sets into a manageable list of candidates, identifying novel targets for future analysis.
AU  - Mcdonald,J
AU  - Kaforou,M
AU  - Clare,S
AU  - Hale,C
AU  - Ivanova,M
AU  - Huntley,D
AU  - Dorner,M
AU  - Wright,VJ
AU  - levin,M
AU  - Torres,FM
AU  - Herberg,J
AU  - Tregoning,JS
DO  - 10.1128/mSystems.00051-16
PY  - 2016///
SN  - 2379-5077
TI  - A Simple Screening Approach To Prioritize Genes for Functional Analysis Identifies a Role for Interferon Regulatory Factor 7 in the Control of Respiratory Syncytial Virus Disease
T2  - mSystems
UR  - http://dx.doi.org/10.1128/mSystems.00051-16
UR  - http://hdl.handle.net/10044/1/33166
VL  - 1
ER  -
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