151 results found
Cai S, Sze JYY, Ivanov AP, et al., 2019, Small molecule electro-optical binding assay using nanopores., Nat Commun, Vol: 10
The identification of short nucleic acids and proteins at the single molecule level is a major driving force for the development of novel detection strategies. Nanopore sensing has been gaining in prominence due to its label-free operation and single molecule sensitivity. However, it remains challenging to detect small molecules selectively. Here we propose to combine the electrical sensing modality of a nanopore with fluorescence-based detection. Selectivity is achieved by grafting either molecular beacons, complementary DNA, or proteins to a DNA molecular carrier. We show that the fraction of synchronised events between the electrical and optical channels, can be used to perform single molecule binding assays without the need to directly label the analyte. Such a strategy can be used to detect targets in complex biological fluids such as human serum and urine. Future optimisation of this technology may enable novel assays for quantitative protein detection as well as gene mutation analysis with applications in next-generation clinical sample analysis.
Xue L, Cadinu P, Nadappuram BP, et al., 2018, Gated Single-Molecule Transport in Double-Barreled Nanopores, ACS APPLIED MATERIALS & INTERFACES, Vol: 10, Pages: 38621-38629, ISSN: 1944-8244
Ma Y, Zagar C, Klemme DJ, et al., 2018, A Tunable Nanoplasmonic Mirror at an Electrochemical Interface, ACS PHOTONICS, Vol: 5, Pages: 4604-4616, ISSN: 2330-4022
Al Sulaiman D, Cadinu P, Ivanov AP, et al., 2018, Chemically Modified Hydrogel-Filled Nanopores: A Tunable Platform for Single-Molecule Sensing, NANO LETTERS, Vol: 18, Pages: 6084-6093, ISSN: 1530-6984
Ivanov AP, Edel JB, 2018, Scissoring genes with light, NATURE CHEMISTRY, Vol: 10, Pages: 800-801, ISSN: 1755-4330
Rakers V, Cadinu P, Edel JB, et al., 2018, Development of microfluidic platforms for the synthesis of metal complexes and evaluation of their DNA affinity using online FRET melting assays, CHEMICAL SCIENCE, Vol: 9, Pages: 3459-3469, ISSN: 2041-6520
Cadinu P, Campolo G, Pud S, et al., 2018, Double Barrel Nanopores as a New Tool for Controlling Single-Molecule Transport, NANO LETTERS, Vol: 18, Pages: 2738-2745, ISSN: 1530-6984
HayleyWeir, Edel JB, Kornyshev AA, et al., 2018, Towards Electrotuneable Nanoplasmonic Fabry-Perot Interferometer, SCIENTIFIC REPORTS, Vol: 8, ISSN: 2045-2322
Peveler WJ, Noimark S, Al-Azawi H, et al., 2018, Covalently Attached Antimicrobial Surfaces Using BODIPY: Improving Efficiency and Effectiveness, ACS APPLIED MATERIALS & INTERFACES, Vol: 10, Pages: 98-104, ISSN: 1944-8244
Velleman L, Scarabelli L, Sikdar D, et al., 2017, Monitoring plasmon coupling and SERS enhancement through in situ nanoparticle spacing modulation, FARADAY DISCUSSIONS, Vol: 205, Pages: 67-83, ISSN: 1359-6640
Aizpurua J, Arnolds H, Baumberg J, et al., 2017, Ultrasensitive and towards single molecule SERS: general discussion, FARADAY DISCUSSIONS, Vol: 205, Pages: 291-330, ISSN: 1359-6640
Barik A, Zhang Y, Grassi R, et al., 2017, Graphene-edge dielectrophoretic tweezers for trapping of biomolecules, NATURE COMMUNICATIONS, Vol: 8, ISSN: 2041-1723
Sze JYY, Ivanov AP, Cass AEG, et al., 2017, Single molecule multiplexed nanopore protein screening in human serum using aptamer modified DNA carriers, NATURE COMMUNICATIONS, Vol: 8, ISSN: 2041-1723
Crick CR, Albella P, Kim H-J, et al., 2017, Low-Noise Plasmonic Nanopore Biosensors for Single Molecule Detection at Elevated Temperatures, ACS PHOTONICS, Vol: 4, Pages: 2835-2842, ISSN: 2330-4022
Recently, there has been a drive to design and develop fully tunable metamaterials for applications ranging from new classes of sensors to superlenses among others. Although advances have been made, tuning and modulating the optical properties in real time remains a challenge. We report on the first realization of a reversible electrotunable liquid mirror based on voltage-controlled self-assembly/disassembly of 16 nm plasmonic nanoparticles at the interface between two immiscible electrolyte solutions. We show that optical properties such as reflectivity and spectral position of the absorption band can be varied in situ within ±0.5 V. This observed effect is in excellent agreement with theoretical calculations corresponding to the change in average interparticle spacing. This electrochemical fully tunable nanoplasmonic platform can be switched from a highly reflective 'mirror' to a transmissive 'window' and back again. This study opens a route towards realization of such platforms in future micro/nanoscale electrochemical cells, enabling the creation of tunable plasmonic metamaterials.
Cadinu P, Paulose Nadappuram B, Lee DJ, et al., 2017, Single Molecule Trapping and Sensing Using Dual Nanopores Separated by a Zeptoliter Nanobridge., Nano letters, Vol: 17, Pages: 6376-6384, ISSN: 1530-6984
There is a growing realization, especially within the diagnostic and therapeutic community, that the amount of information enclosed in a single molecule can not only enable a better understanding of biophysical pathways, but also offer exceptional value for early stage biomarker detection of disease onset. To this end, numerous single molecule strategies have been proposed, and in terms of label-free routes, nanopore sensing has emerged as one of the most promising methods. However, being able to finely control molecular transport in terms of transport rate, resolution, and signal-to-noise ratio (SNR) is essential to take full advantage of the technology benefits. Here we propose a novel solution to these challenges based on a method that allows biomolecules to be individually confined into a zeptoliter nanoscale droplet bridging two adjacent nanopores (nanobridge) with a 20 nm separation. Molecules that undergo confinement in the nanobridge are slowed down by up to 3 orders of magnitude compared to conventional nanopores. This leads to a dramatic improvement in the SNR, resolution, sensitivity, and limit of detection. The strategy implemented is universal and as highlighted in this manuscript can be used for the detection of dsDNA, RNA, ssDNA, and proteins.
Ren R, Zhang Y, Nadappuram BP, et al., 2017, Nanopore extended field-effect transistor for selective single-molecule biosensing, NATURE COMMUNICATIONS, Vol: 8, ISSN: 2041-1723
Lauri A, Velleman L, Xiao X, et al., 2017, 3D Confocal Raman Tomography to Probe Field Enhancements inside Supercluster Metamaterials, ACS PHOTONICS, Vol: 4, Pages: 2070-2077, ISSN: 2330-4022
Lin X, Ivanov AP, Edel JB, 2017, Selective single molecule nanopore sensing of proteins using DNA aptamer-functionalised gold nanoparticles, CHEMICAL SCIENCE, Vol: 8, Pages: 3905-3912, ISSN: 2041-6520
Gielen F, Butz M, Rees EJ, et al., 2017, Quantitative Affinity Determination by Fluorescence Anisotropy Measurements of Individual Nanoliter Droplets, ANALYTICAL CHEMISTRY, Vol: 89, Pages: 1092-1101, ISSN: 0003-2700
Panich S, Sleiman MH, Steer I, et al., 2016, Real-Time Monitoring of Ligand Binding to G-Quadruplex and Duplex DNA by Whispering Gallery Mode Sensing, ACS SENSORS, Vol: 1, Pages: 1097-1102, ISSN: 2379-3694
Sikdar D, Hasan SB, Urbakh M, et al., 2016, Unravelling the optical responses of nanoplasmonic mirror-on-mirror metamaterials, PHYSICAL CHEMISTRY CHEMICAL PHYSICS, Vol: 18, Pages: 20486-20498, ISSN: 1463-9076
Bougot-Robin K, Paget J, Atkins SC, et al., 2016, Optimization and Design of an Absorbance Spectrometer Controlled Using a Raspberry Pi To Improve Analytical Skills, JOURNAL OF CHEMICAL EDUCATION, Vol: 93, Pages: 1232-1240, ISSN: 0021-9584
Crick CR, Noimark S, Peveler WJ, et al., 2016, Advanced Compositional Analysis of Nanoparticle-polymer Composites Using Direct Fluorescence Imaging, JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, ISSN: 1940-087X
Freedman KJ, Crick CR, Albella P, et al., 2016, On-Demand Surface- and Tip-Enhanced Raman Spectroscopy Using Dielectrophoretic Trapping and Nanopore Sensing, ACS PHOTONICS, Vol: 3, Pages: 1036-1044, ISSN: 2330-4022
Kumar S, Johnson TW, Wood CK, et al., 2016, Template-Stripped Multifunctional Wedge and Pyramid Arrays for Magnetic Nanofocusing and Optical Sensing, ACS APPLIED MATERIALS & INTERFACES, Vol: 8, Pages: 9319-9326, ISSN: 1944-8244
Edel JB, Kornyshev AA, Kucernak AR, et al., 2016, Fundamentals and applications of self-assembled plasmonic nanoparticles at interfaces, CHEMICAL SOCIETY REVIEWS, Vol: 45, Pages: 1581-1596, ISSN: 0306-0012
Freedman KJ, Otto LM, Ivanov AP, et al., 2016, Nanopore sensing at ultra-low concentrations using single-molecule dielectrophoretic trapping, NATURE COMMUNICATIONS, Vol: 7, ISSN: 2041-1723
Turek VA, Francescato Y, Cadinu P, et al., 2016, Self-Assembled Spherical Supercluster Metamaterials from Nanoscale Building Blocks, ACS PHOTONICS, Vol: 3, Pages: 35-42, ISSN: 2330-4022
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