139 results found
Cadinu P, Paulose Nadappuram B, Lee DJ, et al., 2017, Single Molecule Trapping and Sensing Using Dual Nanopores Separated by a Zeptoliter Nanobridge., Nano letters, ISSN: 1530-6984
There is a growing realization, especially within the diagnostic and therapeutic community, that the amount of information enclosed in a single molecule can not only enable a better understanding of biophysical pathways, but also offer exceptional value for early stage biomarker detection of disease onset. To this end, numerous single molecule strategies have been proposed, and in terms of label-free routes, nanopore sensing has emerged as one of the most promising methods. However, being able to finely control molecular transport in terms of transport rate, resolution, and signal-to-noise ratio (SNR) is essential to take full advantage of the technology benefits. Here we propose a novel solution to these challenges based on a method that allows biomolecules to be individually confined into a zeptoliter nanoscale droplet bridging two adjacent nanopores (nanobridge) with a 20 nm separation. Molecules that undergo confinement in the nanobridge are slowed down by up to 3 orders of magnitude compared to conventional nanopores. This leads to a dramatic improvement in the SNR, resolution, sensitivity, and limit of detection. The strategy implemented is universal and as highlighted in this manuscript can be used for the detection of dsDNA, RNA, ssDNA, and proteins.
Crick CR, Albella P, Kim H-J, et al., 2017, Low-noise Plasmonic Nanopore Biosensors for Single Molecule Detection at Elevated Temperatures, ACS Photonics, ISSN: 2330-4022
Gielen F, Butz M, Rees EJ, et al., 2017, Quantitative Affinity Determination by Fluorescence Anisotropy Measurements of Individual Nanoliter Droplets, ANALYTICAL CHEMISTRY, Vol: 89, Pages: 1092-1101, ISSN: 0003-2700
Lauri A, Velleman L, Xiao X, et al., 2017, 3D Confocal Raman Tomography to Probe Field Enhancements inside Supercluster Metamaterials, ACS PHOTONICS, Vol: 4, Pages: 2070-2077, ISSN: 2330-4022
Lin X, Ivanov AP, Edel JB, 2017, Selective single molecule nanopore sensing of proteins using DNA aptamer-functionalised gold nanoparticles, CHEMICAL SCIENCE, Vol: 8, Pages: 3905-3912, ISSN: 2041-6520
Recently, there has been a drive to design and develop fully tunable metamaterials for applications ranging from new classes of sensors to superlenses among others. Although advances have been made, tuning and modulating the optical properties in real time remains a challenge. We report on the first realization of a reversible electrotunable liquid mirror based on voltage-controlled self-assembly/disassembly of 16 nm plasmonic nanoparticles at the interface between two immiscible electrolyte solutions. We show that optical properties such as reflectivity and spectral position of the absorption band can be varied in situ within ±0.5 V. This observed effect is in excellent agreement with theoretical calculations corresponding to the change in average interparticle spacing. This electrochemical fully tunable nanoplasmonic platform can be switched from a highly reflective 'mirror' to a transmissive 'window' and back again. This study opens a route towards realization of such platforms in future micro/nanoscale electrochemical cells, enabling the creation of tunable plasmonic metamaterials.
Ren R, Zhang Y, Nadappuram BP, et al., 2017, Nanopore extended field-effect transistor for selective single-molecule biosensing, NATURE COMMUNICATIONS, Vol: 8, ISSN: 2041-1723
Velleman L, Scarabelli L, Sikdar D, et al., 2017, Monitoring plasmon coupling and SERS enhancement through in situ nanoparticle spacing modulation., Faraday Discuss, ISSN: 1359-6640
Self-assembled nanoparticle (NP) arrays at liquid interfaces provide a unique optical response which has opened the door to new tuneable metamaterials for sensing and optical applications. NPs can spontaneously assemble at a liquid-liquid interface, forming an ordered, self-healing, low-defect 2D film. The close proximity of the NPs at the interface results in collective plasmonic modes with a spectral response dependent on the distance between the NPs and induces large field enhancements within the gaps. In this study, we assembled spherical and rod-shaped gold NPs with the aim of improving our understanding of NP assembly processes at liquid interfaces, working towards finely controlling their structure and producing tailored optical and enhanced Raman signals. We systematically tuned the assembly and spacing between NPs through increasing or decreasing the degree of electrostatic screening with the addition of electrolyte or pH adjustment. The in situ modulation of the nanoparticle position on the same sample allowed us to monitor plasmon coupling and the resulting SERS enhancement processes in real time, with sub-nm precision.
Bougot-Robin K, Hoste JW, Le Thomas N, et al., 2016, Highly sensitive detection using microring resonator and nanopores, Conference on Optical Sensing and Detection IV, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X
Bougot-Robin K, Paget J, Atkins SC, et al., 2016, Optimization and Design of an Absorbance Spectrometer Controlled Using a Raspberry Pi To Improve Analytical Skills, JOURNAL OF CHEMICAL EDUCATION, Vol: 93, Pages: 1232-1240, ISSN: 0021-9584
Crick CR, Noimark S, Peveler WJ, et al., 2016, Advanced Compositional Analysis of Nanoparticle-polymer Composites Using Direct Fluorescence Imaging, JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, ISSN: 1940-087X
Edel JB, Kornyshev AA, Kucernak AR, et al., 2016, Fundamentals and applications of self-assembled plasmonic nanoparticles at interfaces, CHEMICAL SOCIETY REVIEWS, Vol: 45, Pages: 1581-1596, ISSN: 0306-0012
Elani Y, Solvas XCI, Edel JB, et al., 2016, Microfluidic generation of encapsulated droplet interface bilayer networks (multisomes) and their use as cell-like reactors, CHEMICAL COMMUNICATIONS, Vol: 52, Pages: 5961-5964, ISSN: 1359-7345
Freedman KJ, Crick CR, Albella P, et al., 2016, On-Demand Surface- and Tip-Enhanced Raman Spectroscopy Using Dielectrophoretic Trapping and Nanopore Sensing, ACS PHOTONICS, Vol: 3, Pages: 1036-1044, ISSN: 2330-4022
Freedman KJ, Otto LM, Ivanov AP, et al., 2016, Nanopore sensing at ultra-low concentrations using single-molecule dielectrophoretic trapping, NATURE COMMUNICATIONS, Vol: 7, ISSN: 2041-1723
Kumar S, Johnson TW, Wood CK, et al., 2016, Template-Stripped Multifunctional Wedge and Pyramid Arrays for Magnetic Nanofocusing and Optical Sensing, ACS APPLIED MATERIALS & INTERFACES, Vol: 8, Pages: 9319-9326, ISSN: 1944-8244
Panich S, Sleiman MH, Steer I, et al., 2016, Real-Time Monitoring of Ligand Binding to G-Quadruplex and Duplex DNA by Whispering Gallery Mode Sensing, ACS SENSORS, Vol: 1, Pages: 1097-1102, ISSN: 2379-3694
Sikdar D, Hasan SB, Urbakh M, et al., 2016, Unravelling the optical responses of nanoplasmonic mirror-on-mirror metamaterials, PHYSICAL CHEMISTRY CHEMICAL PHYSICS, Vol: 18, Pages: 20486-20498, ISSN: 1463-9076
Turek VA, Francescato Y, Cadinu P, et al., 2016, Self-Assembled Spherical Supercluster Metamaterials from Nanoscale Building Blocks, ACS PHOTONICS, Vol: 3, Pages: 35-42, ISSN: 2330-4022
Velleman L, Sikdar D, Turek VA, et al., 2016, Tuneable 2D self-assembly of plasmonic nanoparticles at liquid| liquid interfaces, NANOSCALE, Vol: 8, Pages: 19229-19241, ISSN: 2040-3364
Crick CR, Noimark S, Peveler WJ, et al., 2015, Advanced analysis of nanoparticle composites - a means toward increasing the efficiency of functional materials, RSC ADVANCES, Vol: 5, Pages: 53789-53795, ISSN: 2046-2069
Crick CR, Sze JYY, Rosillo-Lopez M, et al., 2015, Selectively Sized Graphene-Based Nanopores for in Situ Single Molecule Sensing, ACS APPLIED MATERIALS & INTERFACES, Vol: 7, Pages: 18188-18194, ISSN: 1944-8244
Edel J, Oh S-H, 2015, Editorial - From nanopores to nanochannels, ANALYST, Vol: 140, Pages: 4732-4732, ISSN: 0003-2654
Ivanov AP, Actis P, Jönsson P, et al., 2015, On-Demand Delivery of Single DNA Molecules Using Nanopipettes, ACS Nano, Vol: 9, Pages: 3587-3595, ISSN: 1936-086X
Kang D-K, Gong X, Cho S, et al., 2015, 3D Droplet Microfluidic Systems for High-Throughput Biological Experimentation, ANALYTICAL CHEMISTRY, Vol: 87, Pages: 10770-10778, ISSN: 0003-2700
Kang DK, Gong X, Cho S, et al., 2015, 3D droplet microfluidic system for high-throughput biological assay, Pages: 897-899
© 15CBMS-0001. Herein, we describe the development of a multilayer droplet microfluidic system for creating concentration gradients and generating microdroplets of varying composition for high-throughput biochemical and cell-based screening applications. The 3D droplet-based microfluidic device consists of multiple PDMS layers, which are used to generate logarithmic concentration gradient reagent profiles. Parallel flow focusing structures are used to form picoliter-sized droplets of defined volumes but of varying composition. As proof of concept, we demonstrate rapid enzymatic activity assays and drug cytotoxicity assays on bacteria. The 3D droplet-based microfluidic platform has the potential to allow for high-efficiency and high-throughput analysis, overcoming the structural limitations of single layer microfluidic systems.
Kucernak ARJ, edel, turek, et al., 2015, Document METHOD OF DETECTING AN ANALYTE IN A SAMPLE USING RAMAN SPECTROSCOPY, INFRA RED SPECTROSCOPY AND/OR FLUORESCENCE SPECTROSCOPY | [VERFAHREN ZUM NACHWEIS EINES ANALYTEN IN EINER PROBE MITTELS RAMAN-SPEKTROSKOPIE, INFRAROTSPEKTROSKOPIE UND/ODER FLUORESZENZSPEKTROSKOPIE], EP2880425
Pitchford WH, Kim H-J, Ivanov AP, et al., 2015, Synchronized Optical and Electronic Detection of Biomolecules Using a Low Noise Nanopore Platform, ACS NANO, Vol: 9, Pages: 1740-1748, ISSN: 1936-0851
Rutkowska A, Freedman K, Skalkowska J, et al., 2015, Electrodeposition and Bipolar Effects in Metallized Nanopores and Their Use in the Detection of Insulin, ANALYTICAL CHEMISTRY, Vol: 87, Pages: 2337-2344, ISSN: 0003-2700
Sze JYY, Kumar S, Ivanov AP, et al., 2015, Fine tuning of nanopipettes using atomic layer deposition for single molecule sensing, ANALYST, Vol: 140, Pages: 4828-4834, ISSN: 0003-2654
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