Imperial College London

Professor Joshua B. Edel

Faculty of Natural SciencesDepartment of Chemistry

Professor of Biosensing & Analytical Sciences
 
 
 
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Contact

 

+44 (0)20 7594 0754joshua.edel Website

 
 
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Assistant

 

Mr John Murrell +44 (0)20 7594 2845

 
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Location

 

442AChemistrySouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Al:2018:10.1021/acs.nanolett.8b03111,
author = {Al, Sulaiman D and Cadinu, P and Ivanov, AP and Edel, JB and Ladame, S},
doi = {10.1021/acs.nanolett.8b03111},
journal = {Nano Letters},
pages = {6084--6093},
title = {Chemically modified hydrogel-filled nanopores: a tunable platform for single-molecule sensing},
url = {http://dx.doi.org/10.1021/acs.nanolett.8b03111},
volume = {18},
year = {2018}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Label-free, single-molecule sensing is anideal candidate for biomedical applications that rely on the detection of low copy numbers in small volumes and potentially complex biofluids. Among them, solid-state nanopores can be engineered to detect single molecules of charged analytes when they are electrically driven through the nanometer-sized aperture. When successfully applied to nucleic acid sensing, fast transport in the range of 10–100 nucleotides per nanosecond often precludes the use of standard nanopores for the detection of the smallest fragments. Herein, hydrogel-filled nanopores (HFN) are reported that combine quartz nanopipettes with biocompatible chemical poly(vinyl) alcohol hydrogels engineered in-house. Hydrogels were modified physically or chemically to finely tune, in a predictable manner, the transport of specific molecules. Controlling the hydrogel mesh size and chemical composition allowed us to slow DNA transport by 4 orders of magnitude and to detect fragments as small as 100 base pairs (bp) with nanopores larger than 20 nm at an ionic strength comparable to physiological conditions. Considering the emergence of cell-free nucleic acids as blood biomarkers for cancer diagnostics or prenatal testing, the successful sensing and size profiling of DNA fragments ranging from 100 bp to >1 kbp long under physiological conditions demonstrates the potential of HFNs as a new generation of powerful and easily tunable molecular diagnostics tools.
AU - Al,Sulaiman D
AU - Cadinu,P
AU - Ivanov,AP
AU - Edel,JB
AU - Ladame,S
DO - 10.1021/acs.nanolett.8b03111
EP - 6093
PY - 2018///
SN - 1530-6984
SP - 6084
TI - Chemically modified hydrogel-filled nanopores: a tunable platform for single-molecule sensing
T2 - Nano Letters
UR - http://dx.doi.org/10.1021/acs.nanolett.8b03111
UR - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000444793500102&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR - http://hdl.handle.net/10044/1/63988
VL - 18
ER -