Imperial College London

Professor Joshua B. Edel

Faculty of Natural SciencesDepartment of Chemistry

Professor of Biosensing & Analytical Sciences
 
 
 
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Contact

 

+44 (0)20 7594 0754joshua.edel Website

 
 
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Assistant

 

Mrs Althea Hartley-Forbes +44 (0)20 7594 5717

 
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Location

 

442AChemistrySouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Casadevall:2011:10.3791/3437,
author = {Casadevall, i Solvas X and Niu, X and Leeper, K and Cho, S and Chang, S-I and Edel, JB and deMello, AJ},
doi = {10.3791/3437},
journal = {J Vis Exp},
title = {Fluorescence detection methods for microfluidic droplet platforms.},
url = {http://dx.doi.org/10.3791/3437},
year = {2011}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - The development of microfluidic platforms for performing chemistry and biology has in large part been driven by a range of potential benefits that accompany system miniaturisation. Advantages include the ability to efficiently process nano- to femoto- liter volumes of sample, facile integration of functional components, an intrinsic predisposition towards large-scale multiplexing, enhanced analytical throughput, improved control and reduced instrumental footprints. In recent years much interest has focussed on the development of droplet-based (or segmented flow) microfluidic systems and their potential as platforms in high-throughput experimentation. Here water-in-oil emulsions are made to spontaneously form in microfluidic channels as a result of capillary instabilities between the two immiscible phases. Importantly, microdroplets of precisely defined volumes and compositions can be generated at frequencies of several kHz. Furthermore, by encapsulating reagents of interest within isolated compartments separated by a continuous immiscible phase, both sample cross-talk and dispersion (diffusion- and Taylor-based) can be eliminated, which leads to minimal cross-contamination and the ability to time analytical processes with great accuracy. Additionally, since there is no contact between the contents of the droplets and the channel walls (which are wetted by the continuous phase) absorption and loss of reagents on the channel walls is prevented. Once droplets of this kind have been generated and processed, it is necessary to extract the required analytical information. In this respect the detection method of choice should be rapid, provide high-sensitivity and low limits of detection, be applicable to a range of molecular species, be non-destructive and be able to be integrated with microfluidic devices in a facile manner. To address this need we have developed a suite of experimental tools and protocols that enable the extraction of large amounts of photophysical info
AU - Casadevall,i Solvas X
AU - Niu,X
AU - Leeper,K
AU - Cho,S
AU - Chang,S-I
AU - Edel,JB
AU - deMello,AJ
DO - 10.3791/3437
PY - 2011///
TI - Fluorescence detection methods for microfluidic droplet platforms.
T2 - J Vis Exp
UR - http://dx.doi.org/10.3791/3437
UR - https://www.ncbi.nlm.nih.gov/pubmed/22215381
ER -