Imperial College London

Professor Joshua B. Edel

Faculty of Natural SciencesDepartment of Chemistry

Professor of Biosensing & Analytical Sciences
 
 
 
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Contact

 

+44 (0)20 7594 0754joshua.edel Website

 
 
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Assistant

 

Mr John Murrell +44 (0)20 7594 2845

 
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Location

 

442AChemistrySouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Panich:2016:10.1021/acssensors.6b00301,
author = {Panich, S and Sleiman, MH and Steer, I and Ladame, S and Edel, JB},
doi = {10.1021/acssensors.6b00301},
journal = {ACS Sensors},
pages = {1097--1102},
title = {Real-Time Monitoring of Ligand Binding to G-Quadruplex and Duplex DNA by Whispering Gallery Mode Sensing},
url = {http://dx.doi.org/10.1021/acssensors.6b00301},
volume = {1},
year = {2016}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - The therapeutic potential of small molecules targeting G-quadruplexes has gained credibility since such structures were shown to form in human cells and to be highly prevalent in the human genome, most notably at telomere ends and in oncogene promoters. Herein, we perform whispering gallery mode (WGM) sensing for monitoring DNA–small molecule interactions. Unlike most existing technologies, WGM sensing offers numerous advantages including high sensitivity, real-time analysis, easy access to kinetic parameters, and much lower cost than current gold standards. In this work, interactions of five known DNA-binding ligands with either G-quadruplex or duplex DNA immobilized on a sphere microresonator have been assessed. The induced shift of the resonant mode from quadruplex (or duplex)–ligand binding was used to estimate kinetic parameters. Association and dissociation rate constants (kon and koff, respectively) as well as dissociation equilibrium constants (KD) were measured for these five ligands binding to both duplex and quadruplex DNA.
AU - Panich,S
AU - Sleiman,MH
AU - Steer,I
AU - Ladame,S
AU - Edel,JB
DO - 10.1021/acssensors.6b00301
EP - 1102
PY - 2016///
SN - 2379-3694
SP - 1097
TI - Real-Time Monitoring of Ligand Binding to G-Quadruplex and Duplex DNA by Whispering Gallery Mode Sensing
T2 - ACS Sensors
UR - http://dx.doi.org/10.1021/acssensors.6b00301
UR - http://hdl.handle.net/10044/1/41454
VL - 1
ER -