Imperial College London

Professor Joshua B. Edel

Faculty of Natural SciencesDepartment of Chemistry

Professor of Biosensing & Analytical Sciences
 
 
 
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Contact

 

+44 (0)20 7594 0754joshua.edel Website

 
 
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Assistant

 

Mr John Murrell +44 (0)20 7594 2845

 
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Location

 

442AChemistrySouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Lin:2017:10.1039/C7SC00415J,
author = {Lin, X and Ivanov, AP and Edel, JB},
doi = {10.1039/C7SC00415J},
journal = {Chemical Science},
pages = {3905--3912},
title = {Selective single molecule nanopore sensing of proteins using DNA aptamer-functionalised gold nanoparticles},
url = {http://dx.doi.org/10.1039/C7SC00415J},
volume = {8},
year = {2017}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Single molecule detection methods, such as nanopore sensors have found increasing importance in applications ranging from gaining a better understanding of biophysical processes to technology driven solutions such as DNA sequencing. However, challenges remain especially in relation to improving selectivity to probe specific targets or to alternatively enable detection of smaller molecules such as small-sized proteins with a sufficiently high signal-to-noise ratio. In this article, we propose a solution to these technological challenges by using DNA aptamer-modified gold nanoparticles (AuNPs) that act as a molecular carrier through the nanopore sensor. We show that this approach offers numerous advantages including: high levels of selectivity, efficient capture from a complex mixture, enhanced signal, minimized analyte-sensor surface interactions, and finally can be used to enhance the event detection rate. This is demonstrated by incorporating a lysozyme binding aptamer to a 5 nm AuNP carrier to selectively probe lysozyme within a cocktail of proteins. We show that nanopores can reveal sub-complex molecular information, by discriminating the AuNP from the protein analyte, indicating the potential use of this technology for single molecule analysis of different molecular analytes specifically bound to AuNP.
AU - Lin,X
AU - Ivanov,AP
AU - Edel,JB
DO - 10.1039/C7SC00415J
EP - 3912
PY - 2017///
SN - 2041-6539
SP - 3905
TI - Selective single molecule nanopore sensing of proteins using DNA aptamer-functionalised gold nanoparticles
T2 - Chemical Science
UR - http://dx.doi.org/10.1039/C7SC00415J
UR - http://hdl.handle.net/10044/1/45655
VL - 8
ER -