Imperial College London

Professor Joshua B. Edel

Faculty of Natural SciencesDepartment of Chemistry

Professor of Biosensing & Analytical Sciences
 
 
 
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Contact

 

+44 (0)20 7594 0754joshua.edel Website

 
 
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Assistant

 

Mr John Murrell +44 (0)20 7594 2845

 
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Location

 

442AChemistrySouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Rakers:2018:10.1039/c8sc00528a,
author = {Rakers, V and Cadinu, P and Edel, JB and Vilar, R},
doi = {10.1039/c8sc00528a},
journal = {Chemical Science},
pages = {3459--3469},
title = {Development of microfluidic platforms for the synthesis of metal complexes and evaluation of their DNA affinity using online FRET melting assays},
url = {http://dx.doi.org/10.1039/c8sc00528a},
volume = {9},
year = {2018}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Guanine-rich DNA sequences can fold into quadruple-stranded structures known as G-quadruplexes. These structures have been proposed to play important biological roles and have been identified as potential drug targets. As a result, there is increasing interest in developing small molecules that can bind to G-quadruplexes. So far, these efforts have been mostly limited to conventional batch synthesis. Furthermore, no quick on-line method to assess new G-quadruplex binders has been developed. Herein, we report on two new microfluidic platforms to: (a) readily prepare G-quadruplex binders (based on metal complexes) in flow, quantitatively and without the need for purification before testing; (b) a microfluidic platform (based on FRET melting assays of DNA) that enables the real-time and on-line assessment of G-quadruplex binders in continuous flow.
AU - Rakers,V
AU - Cadinu,P
AU - Edel,JB
AU - Vilar,R
DO - 10.1039/c8sc00528a
EP - 3469
PY - 2018///
SN - 2041-6520
SP - 3459
TI - Development of microfluidic platforms for the synthesis of metal complexes and evaluation of their DNA affinity using online FRET melting assays
T2 - Chemical Science
UR - http://dx.doi.org/10.1039/c8sc00528a
UR - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000429203500005&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR - http://hdl.handle.net/10044/1/60210
VL - 9
ER -