Publications
188 results found
Cho S, Kang DK, Sim S, et al., 2013, A Droplet-Based Microfluidic Platform for High-Throughput, Multi-Parameter Screening of Photosensitizer Activity, Analytical Chemistry
Draper MC, Crick CR, Orlickaite V, et al., 2013, Superhydrophobic Surfaces as an On-Chip Microfluidic Toolkit for Total Droplet Control, Vol: 85, Pages: 5405-5410-5405-5410, ISSN: 0003-2700
Freedman KJ, Haq R, Edel JB, et al., 2013, Single molecule unfolding and stretching of protein domains inside a solid-state nanopore by electric field, Scientific Reports, Vol: 3
Gielen F, Van Vliet L, Koprowski BT, et al., 2013, A Fully Unsupervised Compartment-on-demand Platform for Precise Nanolitre Assays of Time-Dependent Steady-State Enzyme Kinetics and Inhibition, Analytical Chemistry, Vol: 85, Pages: 4761-4769, ISSN: 0003-2700
The ability to miniaturize biochemical assays in water-in-oil emulsion droplets allows a massive scale-down of reaction volumes, so that high-throughput experimentation can be performed more economically and more efficiently. Generating such droplets in compartment-on-demand (COD) platforms is the basis for rapid, automated screening of chemical and biological libraries with minimal volume consumption. Herein, we describe the implementation of such a COD platform to perform high precision nanoliter assays. The coupling of a COD platform to a droplet absorbance detection set-up results in a fully automated analytical system. Michaelis–Menten parameters of 4-nitrophenyl glucopyranoside hydrolysis by sweet almond β-glucosidase can be generated based on 24 time-courses taken at different substrate concentrations with a total volume consumption of only 1.4 μL. Importantly, kinetic parameters can be derived in a fully unsupervised manner within 20 min: droplet production (5 min), initial reading of the droplet sequence (5 min), and droplet fusion to initiate the reaction and read-out over time (10 min). Similarly, the inhibition of the enzymatic reaction by conduritol B epoxide and 1-deoxynojirimycin was measured, and Ki values were determined. In both cases, the kinetic parameters obtained in droplets were identical within error to values obtained in titer plates, despite a >104-fold volume reduction, from micro- to nanoliters.
Panich S, Wilson K, Edel JB, 2013, Trace heavy metal analysis using whispering gallery mode sensing, Pages: 1556-1558
A Whispering Gallery Mode (WGM) resonator assay for the detection of trace levels of Pb2+ was demonstrated. The platform consists of a glass microspheres decorated with glutathione (GSH)-modified gold nanoparticles (GNPs). In our study, GSH was used as a chelator to provide high specificity to Pb2+. When the surface of the microsphere was exposed to Pb 2+ with the assistance of a fluidic cell, the resonant mode shifts to longer wavelengths. The shift is found to be proportional to the concentration of Pb2+ at concentrations as low as 10 ppt making this technique versatile for portable in-field environmental monitoring.
Edel JB, Albrecht T, 2013, Engineered Nanopores for Bioanalytical Applications: A Volume in Micro and Nano Technologies, ISBN: 9781437734737
Engineered Nanopores for Bioanalytical Applications is the first book to focus primarily on practical analytical applications of nanopore development. These nanoscale analytical techniques have exciting potential because they can be used in applications such as DNA sequencing, DNA fragment sizing, DNA/protein binding, and protein/protein binding. This book provides a solid professional reference on nanopores for readers in academia, industry and engineering and biomedical fields. In addition, the book describes the instrumentation, fabrication, and experimental methods necessary to carry out nanopore-based experiments for developing new devices. © 2013 Elsevier Inc. All rights reserved.
Cecchini MP, Wiener A, Turek V, et al., 2013, Rapid Ultra-Sensitive Single Particle Surface Enhanced Raman Spectroscopy using Metallic Nanopores, Nano Letters
Japrung D, Dogan J, Freedman KJ, et al., 2013, Single Molecule Studies of Intrinsically Disordered Proteins Using Solid-State Nanopores, Analytical Chemistry
Rutkowska A, Edel JB, Albrecht T, 2013, Mapping the Ion Current Distribution in Nanopore/Electrode Devices, ACS Nano
Cecchini MP, Turek VA, Paget J, et al., 2013, Self-assembled nanoparticle arrays for multiphase trace analyte detection, Nature Materials
Miles BN, Ivanov AP, Wilson KA, et al., 2013, Single molecule sensing with solid-state nanopores: novel materials, methods, and applications, Chemical Society Reviews, Vol: 42, Pages: 15-28
Albrecht T, Edel JB, 2013, Engineered Nanopores for Bioanalytical Applications Introduction, ENGINEERED NANOPORES FOR BIOANALYTICAL APPLICATIONS, Editors: Edel, Albrecht, Publisher: WILLIAM ANDREW INC, Pages: IX-XI
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- Citations: 19
Draper MC, Niu X, Cho S, et al., 2012, Compartmentalization of Electrophoretically Separated Analytes in a Multiphase Microfluidic Platform, ANALYTICAL CHEMISTRY, Vol: 84, Pages: 5801-5808, ISSN: 0003-2700
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- Citations: 17
Gielen F, deMello AJ, Edel JB, 2012, Dielectric Cell Response in Highly Conductive Buffers, ANALYTICAL CHEMISTRY, Vol: 84, Pages: 1849-1853, ISSN: 0003-2700
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- Citations: 11
Cho S, Kang DK, Sim S, et al., 2012, A droplet-based microfluidic system for high- Throughput screening of photosensitisers against microbial organisms, Pages: 332-334
Herein, we present a modular droplet-basecl microfluidic approach for performing liigh throughput cytotoxicity screening of photo sensitizers against microbial organisms. Multiple novel fluidic operation modalities such as large-scale chamber based light irradiation, reinjection and low voltage driven electrocoalescence are introduced. Also, photosensitiser drug cytotoxicity on E.coli cells are evaluated in microfluidic device using fluorescent viability assay indicator and compared with conventional colony forming unit counting cytotoxicity assay.
Turek V, Cecchini MP, Paget J, et al., 2012, A Plasmonic Ruler at the Liquid-Liquid Interface, ACS Nano
Hassan S, Gielen F, Niu X, et al., 2012, Controlled one dimensional oscillation of the Belousov–Zhabotinsky reaction confined within microchannels, Rsc Advances, Vol: 2, Pages: 6408-6410
Kim J-Y, Cho S-W, Kang D-K, et al., 2012, Lab-chip HPLC with integrated droplet-based microfluidics for separation and high frequency compartmentalisation, CHEMICAL COMMUNICATIONS, Vol: 48, Pages: 9144-9146, ISSN: 1359-7345
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- Citations: 19
Elvira KS, Leatherbarrow R, Edel JB, et al., 2012, Droplet dispensing in digital microfluidic devices: Assessment of long-term reproducibility, Biomicrofluidics
Casadevall i Solvas X, Prodromakis T, Turek V, et al., 2012, Microfluidic evaporator for on-chip sample concentration, Lab on A Chip
Casadevall i Solvas X, Niu X, Leeper K, et al., 2011, Fluorescence detection methods for microfluidic droplet platforms., J Vis Exp
The development of microfluidic platforms for performing chemistry and biology has in large part been driven by a range of potential benefits that accompany system miniaturisation. Advantages include the ability to efficiently process nano- to femoto- liter volumes of sample, facile integration of functional components, an intrinsic predisposition towards large-scale multiplexing, enhanced analytical throughput, improved control and reduced instrumental footprints. In recent years much interest has focussed on the development of droplet-based (or segmented flow) microfluidic systems and their potential as platforms in high-throughput experimentation. Here water-in-oil emulsions are made to spontaneously form in microfluidic channels as a result of capillary instabilities between the two immiscible phases. Importantly, microdroplets of precisely defined volumes and compositions can be generated at frequencies of several kHz. Furthermore, by encapsulating reagents of interest within isolated compartments separated by a continuous immiscible phase, both sample cross-talk and dispersion (diffusion- and Taylor-based) can be eliminated, which leads to minimal cross-contamination and the ability to time analytical processes with great accuracy. Additionally, since there is no contact between the contents of the droplets and the channel walls (which are wetted by the continuous phase) absorption and loss of reagents on the channel walls is prevented. Once droplets of this kind have been generated and processed, it is necessary to extract the required analytical information. In this respect the detection method of choice should be rapid, provide high-sensitivity and low limits of detection, be applicable to a range of molecular species, be non-destructive and be able to be integrated with microfluidic devices in a facile manner. To address this need we have developed a suite of experimental tools and protocols that enable the extraction of large amounts of photophysical info
Chansin GAT, Hong J, Dusting J, et al., 2011, Resizing Metal-Coated Nanopores Using a Scanning Electron Microscope, SMALL, Vol: 7, Pages: 2736-2741, ISSN: 1613-6810
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- Citations: 5
Freedman KJ, Jurgens M, Prabhu A, et al., 2011, Chemical, Thermal, and Electric Field Induced Unfolding of Single Protein Molecules Studied Using Nanopores, ANALYTICAL CHEMISTRY, Vol: 83, Pages: 5137-5144, ISSN: 0003-2700
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- Citations: 105
Cecchini MP, Hong J, Lim C, et al., 2011, Ultra-Fast Surface Enhanced Resonance Raman Scattering (SERRS) Detection in Droplet-Based Microfluidic Systems, Analytical Chemistry
Cecchini MP, Stapountzi MA, McComb DW, et al., 2011, Flow-Based Autocorrelation Studies for the Detection and Investigation of Single-Particle Surface-Enhanced Resonance Raman Spectroscopic Events, Analytical Chemistry
Niu X, Gielen F, Edel JB, et al., 2011, A microdroplet dilutor for high-throughput screening, Nature Chemistry
Srisa-Art M, deMello AJ, Edel JB, 2010, High-Efficiency Single-Molecule Detection within Trapped Aqueous Microdroplets, JOURNAL OF PHYSICAL CHEMISTRY B, Vol: 114, Pages: 15766-15772, ISSN: 1520-6106
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- Citations: 29
Ivanov AP, Instuli E, McGilvery C, et al., 2010, DNA tunneling detector embedded in a nanopore, Nano Letters, Vol: 11, Pages: 279-285, ISSN: 1530-6992
We report on the fabrication and characterization of a DNA nanopore detector with integrated tunneling electrodes. Functional tunneling devices were identified by tunneling spectroscopy in different solvents and then used in proof-of-principle experiments demonstrating, for the first time, concurrent tunneling detection and ionic current detection of DNA molecules in a nanopore platform. This is an important step toward ultrafast DNA sequencing by tunneling.
Ayub M, Ivanov A, Instuli E, et al., 2010, Nanopore/electrode structures for single molecule biosensing, Pages: 15-18
In this contribution we present some of our recent work on solid-state nanopores, the fabrication of nanopore/electrode structures and how these can be used for single-molecule biosensing applications (DNA, proteins etc.). Examples include bi-potentiostatic experiments with Au/Si3N4 nanopore membranes and a new way of fabricating small metal nanopores with diameters below 20 nm and well-defined pore conductance, based on electrodeposition and ion-current feedback control.
Park S-M, Huh YS, Szeto K, et al., 2010, Rapid Prototyping of Nanofluidic Systems Using Size-Reduced Electrospun Nanofibers for Biomolecular Analysis, SMALL, Vol: 6, Pages: 2420-2426, ISSN: 1613-6810
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- Citations: 14
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