Professor Joshua B. Edel

Vijayakumar K, Gulati S, deMello AJ, Edel JBet al., 2010, Rapid cell extraction in aqueous two-phase microdroplet systems, CHEMICAL SCIENCE, Vol: 1, Pages: 447-452, ISSN: 2041-6520

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• Citations: 62

Gielen F, Pereira F, deMello AJ, Edel JBet al., 2010, High-Resolution Local Imaging of Temperature in Dielectrophoretic Platforms, ANALYTICAL CHEMISTRY, Vol: 82, Pages: 7509-7514, ISSN: 0003-2700

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• Citations: 17

Solvas XCI, Srisa-Art M, demello AJ, Edel JBet al., 2010, Mapping of Fluidic Mixing in Microdroplets with 1 μs Time Resolution Using Fluorescence Lifetime Imaging, ANALYTICAL CHEMISTRY, Vol: 82, Pages: 3950-3956, ISSN: 0003-2700

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• Citations: 37

Ayub M, Ivanov A, Hong J, Kuhn P, Instuli E, Edel JB, Albrecht Tet al., 2010, Precise electrochemical fabrication of sub-20 nm solid-state nanopores for single-molecule biosensing, Journal of Physics: Condensed Matter, Vol: 22, Pages: 454128-454128

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Khongkow M, Instuli E, Ivanov A, Edel JB, Albrecht Tet al., 2010, Solid-state nanopores: a new tool for biomedical diagnostics

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Ayub M, Ivanov A, Instuli E, Cecchini M, Chansin G, McGilvery C, Hong J, Baldwin G, McComb D, Edel JB, otherset al., 2010, Nanopore/electrode structures for single-molecule biosensing, Electrochimica Acta, Vol: 55, Pages: 8237-8243

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Stanley CE, Elvira KS, Niu XZ, Gee AD, Ces O, Edel JB, deMello AJet al., 2010, A microfluidic approach for high-throughput droplet interface bilayer (DIB) formation, CHEMICAL COMMUNICATIONS, Vol: 46, Pages: 1620-1622, ISSN: 1359-7345

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• Citations: 71

Hong J, Choi M, Edel JB, deMello AJet al., 2010, Passive self-synchronized two-droplet generation, LAB ON A CHIP, Vol: 10, Pages: 2702-2709, ISSN: 1473-0197

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• Citations: 41

Albrecht T, Edel JB, Winterhalter M, 2010, New developments in nanopore research—from fundamentals to applications (Preface), Journal of Physics: Condensed Matter, Vol: 22

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DeMello AJ, French PMW, Neil MAA, Edel JB, Benninger RKP, Bradley DDC, Robinson T, Srisa-art M, Hofmann O, DeMello JCet al., 2009, Optical detection in microfluidics: From the small to the large, Pages: 712-717

Herein we discuss two broad approaches for performing high sensitivity optical detection within microfluidic environments. First, we describe recent work in which fluorescence lifetime imaging has been shown to be a sensitive probe of environmental parameters such as pH, viscosity, molecular concentration and temperature. Additionally, we demonstrate how dynamic fluorescence lifetime imaging can be used to probe mixing dynamics in segmented-flow microfluidic systems. Moreover, we describe recent work at Imperial College London in which semiconducting polymer light emitting diodes and polymer photodetectors are integrated with microfluidic systems to define a novel format for point-of-care diagnostics. ©2009 IEEE.

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Hong J, Choi M, deMello AJ, Edel JBet al., 2009, Interfacial Tension-Mediated Droplet Fusion in Rectangular Microchannels, BIOCHIP JOURNAL, Vol: 3, Pages: 203-207, ISSN: 1976-0280

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• Citations: 13

Niu X, Gielen F, deMello AJ, Edel JBet al., 2009, Electro-Coalescence of Digitally Controlled Droplets, ANALYTICAL CHEMISTRY, Vol: 81, Pages: 7321-7325, ISSN: 0003-2700

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• Citations: 72

Gulati S, Rouilly V, Niu X, Chappell J, Kitney RI, Edel JB, Freemont PS, deMello AJet al., 2009, Opportunities for microfluidic technologies in synthetic biology, JOURNAL OF THE ROYAL SOCIETY INTERFACE, Vol: 6, ISSN: 1742-5689

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• Citations: 54

Srisa-Art M, Kang D-K, Hong J, Park H, Leatherbarrow RJ, Edel JB, Chang S-I, deMello AJet al., 2009, Analysis of Protein-Protein Interactions by Using Droplet-Based Microfluidics, CHEMBIOCHEM, Vol: 10, Pages: 1605-1611, ISSN: 1439-4227

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• Citations: 55

Edel JB, Ayub M, Albrecht T, 2009, Electrically gated nanopores for single molecule separation, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Vol: 237, Pages: 175-175, ISSN: 0065-7727

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Gielen F, deMello AJ, Cass T, Edel JBet al., 2009, Increasing the Trapping Efficiency of Particles in Microfluidic Planar Platforms by Means of Negative Dielectrophoresis, JOURNAL OF PHYSICAL CHEMISTRY B, Vol: 113, Pages: 1493-1500, ISSN: 1520-6106

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• Citations: 10

Hong J, Edel JB, deMello AJ, 2009, Micro- and nanofluidic systems for high-throughput biological screening, DRUG DISCOVERY TODAY, Vol: 14, Pages: 134-146, ISSN: 1359-6446

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• Citations: 161

Srisa-Art M, Kang DK, Hong J, Park H, Edel JB, Chang SI, DeMello AJet al., 2009, Analysis of angiogenin-antiangiogenin antibody interactions using droplet-based microfluidics, Pages: 606-608

The manipulation of multi-phase flows in microfluidic systems has been introduced as a fundamental experimental platform for high-throughput experimentation. In this study, we apply fluorescence resonance energy transfer (FRET) measurements in a segmented flow microfluidic platform to the analysis of protein-protein interactions. Angiogenin (ANG) is used as a model protein to confirm the efficacy of our experimental approach. Specifically, an anti-ANG antibody (anti-ANG Ab) and an ANG antigen are labelled with fluorophores to act as donor and acceptor in the FRET measurements. © 2009 CBMS.

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Niu X, Gielen F, DeMello AJ, Edel JBet al., 2009, A hybrid microfluidic chip for digital electro-coalescence of droplets, Pages: 94-96

We describe a universal mechanism for merging multiple aqueous microdroplets within a flowing stream consisting of an oil carrier phase. Our approach involves the use of both a pillar array acting as a passive merging element as well as integrated electrodes acting as an active merging element. The pillar array enables slowing down and trapping of the droplets via the drainage of the oil phase. This brings adjacent droplets into close proximity. At this point, a low electric field is applied to the electrodes which breaks up the thin oil film surrounding the droplets and subsequently results in merging. © 2009 CBMS.

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Stapountzi MA, Edel JB, 2009, Fluorescence Lifetime Imaging within microfluidic structures using a Maximum Likelihood Estimator, Pages: 945-947

A novel technique is developed for visualizing hydrodynamic focusing within microchannels by using Fluorescent Lifetime Imaging (FLIM) along with a Maximum Likelihood Estimator (MLE) adapted from single molecule studies. As little as 10 photons are required to accurately determine fluorescence lifetime and build up a 2-D map of fluorescent lifetimes within a microfluidic device. © 2009 CBMS.

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Ayub M, Hong J, Albrecht T, Edel JBet al., 2009, Electrochemical size control of solid-state nanopores with ionic current feedback, Pages: 1016-1018

Single nanometer-sized pores embedded in an insulating membrane are an exciting new class of nanosensors for rapid electrical detection and characterisation of biomolecules [1]. In most cases, the fabrication of such nanopores requires the high-energy beam of a transmission electron microscope (TEM) or focused ion beam (FIB) tool to drill or reshape a small hole in a freestanding membrane [1,2]. Here, we present a novel method to reduce the size of such pores using electrochemical deposition with ionic current feedback control. Electrophoretic transport of λ-DNA through the electrodeposited nanopores is also demonstrated using electrical detection. © 2009 CBMS.

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Chan KLA, Gulati S, Edel JB, de Mello AJ, Kazarian SGet al., 2009, Chemical imaging of microfluidic flows using ATR-FTIR spectroscopy, LAB ON A CHIP, Vol: 9, Pages: 2909-2913, ISSN: 1473-0197

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• Citations: 103

Srisa-Art M, deMello AJ, Edel JB, 2009, High-throughput confinement and detection of single DNA molecules in aqueous microdroplets, CHEMICAL COMMUNICATIONS, Pages: 6548-6550, ISSN: 1359-7345

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• Citations: 24

Niu XZ, Zhang B, Marszalek RT, Ces O, Edel JB, Klug DR, deMello AJet al., 2009, Droplet-based compartmentalization of chemically separated components in two-dimensional separations, CHEMICAL COMMUNICATIONS, Pages: 6159-6161, ISSN: 1359-7345

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• Citations: 84

Srisa-Art M, Bonzani IC, Williams A, Stevens MM, deMello AJ, Edel JBet al., 2009, Identification of rare progenitor cells from human periosteal tissue using droplet microfluidics, ANALYST, Vol: 134, Pages: 2239-2245, ISSN: 0003-2654

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• Citations: 38

Chansin GAT, Hong J, DeMello AJ, Edel JBet al., 2009, Nanopore-Based Optofluidic Devices for Single Molecule Sensing, NANOFLUIDICS: NANOSCIENCE AND NANOTECHNOLOGY, Editors: Edel, DeMello, Publisher: ROYAL SOC CHEMISTRY, Pages: 139-155, ISBN: 978-0-85404-147-3

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Srisa-Art M, Dyson EC, deMello AJ, Edel JBet al., 2008, Monitoring of real-time streptavidin-biotin binding kinetics using droplet microfluidics, ANALYTICAL CHEMISTRY, Vol: 80, Pages: 7063-7067, ISSN: 0003-2700

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• Citations: 121

Huebner A, Sharma S, Srisa-Art M, Hollfelder F, Edel JB, Demello AJet al., 2008, Microdroplets: a sea of applications?, Lab Chip, Vol: 8, Pages: 1244-1254

The exploitation of microdroplets produced within microfluidic environments has recently emerged as a new and exciting technological platform for applications within the chemical and biological sciences. Interest in microfluidic systems has been stimulated by a range of fundamental features that accompany system miniaturization. Such features include the ability to process and handle small volumes of fluid, improved analytical performance when compared to macroscale analogues, reduced instrumental footprints, low unit cost, facile integration of functional components and the exploitation of atypical fluid dynamics to control molecules in both time and space. Moreover, microfluidic systems that generate and utilize a stream of sub-nanolitre droplets dispersed within an immiscible continuous phase have the added advantage of allowing ultra-high throughput experimentation and being able to mimic conditions similar to that of a single cell (in terms of volume, pH, and salt concentration) thereby compartmentalizing biological and chemical reactions. This review provides an overview of methods for generating, controlling and manipulating droplets. Furthermore, we discuss key fields of use in which such systems may make a significant impact, with particular emphasis on novel applications in the biological and physical sciences.

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Srisa-Art M, deMello AJ, Edel JB, 2008, Fluorescence lifetime imaging of mixing dynamics in continuous-flow microdroplet reactors, PHYSICAL REVIEW LETTERS, Vol: 101, ISSN: 0031-9007

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• Citations: 35

Huebner A, Olguin LF, Bratton D, Whyte G, Huck WTS, de Mello AJ, Edel JB, Abell C, Hollfelder Fet al., 2008, Development of quantitative cell-based enzyme assays in microdroplets, ANALYTICAL CHEMISTRY, Vol: 80, Pages: 3890-3896, ISSN: 0003-2700

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• Citations: 164