Imperial College London
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Professor Joshua B. Edel

Faculty of Natural Sciences, Department of Chemistry

Professor of Biosensing & Analytical Sciences
 
 
 
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Contact

 

+44 (0)20 7594 0754joshua.edel Website

 
 
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Assistant

 

Mr John Murrell +44 (0)20 7594 2845

 
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Location

 

110cMolecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Cai:2019:10.1038/s41467-019-09476-4,
author = {Cai, S and Sze, JYY and Ivanov, AP and Edel, JB},
doi = {10.1038/s41467-019-09476-4},
journal = {Nat Commun},
title = {Small molecule electro-optical binding assay using nanopores.},
url = {http://dx.doi.org/10.1038/s41467-019-09476-4},
volume = {10},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The identification of short nucleic acids and proteins at the single molecule level is a major driving force for the development of novel detection strategies. Nanopore sensing has been gaining in prominence due to its label-free operation and single molecule sensitivity. However, it remains challenging to detect small molecules selectively. Here we propose to combine the electrical sensing modality of a nanopore with fluorescence-based detection. Selectivity is achieved by grafting either molecular beacons, complementary DNA, or proteins to a DNA molecular carrier. We show that the fraction of synchronised events between the electrical and optical channels, can be used to perform single molecule binding assays without the need to directly label the analyte. Such a strategy can be used to detect targets in complex biological fluids such as human serum and urine. Future optimisation of this technology may enable novel assays for quantitative protein detection as well as gene mutation analysis with applications in next-generation clinical sample analysis.
AU  - Cai,S
AU  - Sze,JYY
AU  - Ivanov,AP
AU  - Edel,JB
DO  - 10.1038/s41467-019-09476-4
PY  - 2019///
TI  - Small molecule electro-optical binding assay using nanopores.
T2  - Nat Commun
UR  - http://dx.doi.org/10.1038/s41467-019-09476-4
UR  - https://www.ncbi.nlm.nih.gov/pubmed/30996223
UR  - http://hdl.handle.net/10044/1/69094
VL  - 10
ER  -
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