Imperial College London
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Professor Joshua B. Edel

Faculty of Natural Sciences, Department of Chemistry

Professor of Biosensing & Analytical Sciences
 
 
 
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Contact

 

+44 (0)20 7594 0754joshua.edel Website

 
 
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Assistant

 

Mr John Murrell +44 (0)20 7594 2845

 
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Location

 

110cMolecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Edel:2016:10.1038/ncomms10217,
author = {Edel, JB and Freedman, K and Otto, L and Ivanov, A and Barik, A and Oh, S},
doi = {10.1038/ncomms10217},
journal = {Nature Communications},
pages = {1--9},
title = {Nanopore sensing at ultra-low concentrations using single molecule dielectrophoretic trapping},
url = {http://dx.doi.org/10.1038/ncomms10217},
volume = {7},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Single-molecule techniques are being developed with the exciting prospect of revolutionizing the healthcare industry by generating vast amounts of genetic and proteomic data. One exceptionally promising route is in the use of nanopore sensors. However, a well-known complexity is that detection and capture is predominantly diffusion limited. This problem is compounded when taking into account the capture volume of a nanopore, typically 108–1010 times smaller than the sample volume. To rectify this disproportionate ratio, we demonstrate a simple, yet powerful, method based on coupling single-molecule dielectrophoretic trapping to nanopore sensing. We show that DNA can be captured from a controllable, but typically much larger, volume and concentrated at the tip of a metallic nanopore. This enables the detection of single molecules at concentrations as low as 5 fM, which is approximately a 103 reduction in the limit of detection compared with existing methods, while still maintaining efficient throughput.
AU  - Edel,JB
AU  - Freedman,K
AU  - Otto,L
AU  - Ivanov,A
AU  - Barik,A
AU  - Oh,S
DO  - 10.1038/ncomms10217
EP  - 9
PY  - 2016///
SN  - 2041-1723
SP  - 1
TI  - Nanopore sensing at ultra-low concentrations using single molecule dielectrophoretic trapping
T2  - Nature Communications
UR  - http://dx.doi.org/10.1038/ncomms10217
UR  - https://www.nature.com/articles/ncomms10217
UR  - http://hdl.handle.net/10044/1/28794
VL  - 7
ER  -
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