Imperial College London
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ProfessorJulianJones

Faculty of Engineering, Department of Materials

Professor of Biomaterials
 
 
 
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Contact

 

+44 (0)20 7594 6749julian.r.jones

 
 
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Location

 

207GoldsmithSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{De:2024:10.3390/bioengineering11020112,
author = {De, Mori A and Heyraud, A and Tallia, F and Blunn, G and Jones, JR and Roncada, T and Cobb, J and Al-Jabri, T},
doi = {10.3390/bioengineering11020112},
journal = {Bioengineering (Basel)},
title = {Ovine Mesenchymal Stem Cell Chondrogenesis on a Novel 3D-Printed Hybrid Scaffold In Vitro.},
url = {http://dx.doi.org/10.3390/bioengineering11020112},
volume = {11},
year = {2024}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - This study evaluated the use of silica/poly(tetrahydrofuran)/poly(ε-caprolactone) (SiO2/PTHF/PCL-diCOOH) 3D-printed scaffolds, with channel sizes of either 200 (SC-200) or 500 (SC-500) µm, as biomaterials to support the chondrogenesis of sheep bone marrow stem cells (oBMSC), under in vitro conditions. The objective was to validate the potential use of SiO2/PTHF/PCL-diCOOH for prospective in vivo ovine studies. The behaviour of oBMSC, with and without the use of exogenous growth factors, on SiO2/PTHF/PCL-diCOOH scaffolds was investigated by analysing cell attachment, viability, proliferation, morphology, expression of chondrogenic genes (RT-qPCR), deposition of aggrecan, collagen II, and collagen I (immunohistochemistry), and quantification of sulphated glycosaminoglycans (GAGs). The results showed that all the scaffolds supported cell attachment and proliferation with upregulation of chondrogenic markers and the deposition of a cartilage extracellular matrix (collagen II and aggrecan). Notably, SC-200 showed superior performance in terms of cartilage gene expression. These findings demonstrated that SiO2/PTHF/PCL-diCOOH with 200 µm pore size are optimal for promoting chondrogenic differentiation of oBMSC, even without the use of growth factors.
AU  - De,Mori A
AU  - Heyraud,A
AU  - Tallia,F
AU  - Blunn,G
AU  - Jones,JR
AU  - Roncada,T
AU  - Cobb,J
AU  - Al-Jabri,T
DO  - 10.3390/bioengineering11020112
PY  - 2024///
SN  - 2306-5354
TI  - Ovine Mesenchymal Stem Cell Chondrogenesis on a Novel 3D-Printed Hybrid Scaffold In Vitro.
T2  - Bioengineering (Basel)
UR  - http://dx.doi.org/10.3390/bioengineering11020112
UR  - https://www.ncbi.nlm.nih.gov/pubmed/38391598
VL  - 11
ER  -
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