Imperial College London
Portrait Picture

Dr Julie McDonald

Faculty of Natural Sciences, Department of Life Sciences

Lecturer (MRC-CMBI)
 
 
 
//

Contact

 

+44 (0)20 7594 5247julie.mcdonald Website

 
 
//

Location

 

1.44Flowers buildingSouth Kensington Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Taylor:2020:10.1111/apt.16086,
author = {Taylor, H and Serrano-Contreras, JI and McDonald, JAK and Epstein, J and Fell, JM and Seoane, RC and Li, JV and Marchesi, JR and Hart, AL},
doi = {10.1111/apt.16086},
journal = {Alimentary Pharmacology and Therapeutics},
pages = {1491--1502},
title = {Multiomic features associated with mucosal healing and inflammation in paediatric Crohn's disease},
url = {http://dx.doi.org/10.1111/apt.16086},
volume = {52},
year = {2020}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BACKGROUND: The gastrointestinal microbiota has an important role in mucosal immune homoeostasis and may contribute to maintaining mucosal healing in Crohn's disease (CD). AIM: To identify changes in the microbiota, metabolome and protease activity associated with mucosal healing in established paediatric CD. METHODS: Twenty-five participants aged 3-18 years with CD, disease duration of over 6 months, and maintenance treatment with biological therapy were recruited. They were divided into a low calprotectin group (faecal calprotectin <100 μg/g, "mucosal healing," n = 11), and a high calprotectin group (faecal calprotectin >100 μg/g, "mucosal inflammation," n = 11). 16S gene-based metataxonomics, 1 H-NMR spectroscopy-based metabolic profiling and protease activity assays were performed on stool samples. RESULTS: Relative abundance of Dialister species was six times greater in the low calprotectin group (q = 0.00999). Alpha and beta diversity, total protease activity and inferred metagenomic profiles did not differ between groups. Pentanoate (valerate) and lysine were principal discriminators in a machine-learning model which differentiated high and low calprotectin samples using NMR spectra (R2 0.87, Q2 0.41). Mean relative concentration of pentanoate was 1.35-times greater in the low calprotectin group (95% CI 1.03-1.68, P = 0.036) and was positively correlated with Dialister. Mean relative concentration of lysine was 1.54-times greater in the high calprotectin group (95% CI 1.05-2.03, P = 0.028). CONCLUSIONS: This multiomic study identified an increase in Dialister species and pentanoate, and a decrease in lysine, in patients with "mucosal healing." It supports further investigation of these as potential novel therapeutic targets in CD.
AU  - Taylor,H
AU  - Serrano-Contreras,JI
AU  - McDonald,JAK
AU  - Epstein,J
AU  - Fell,JM
AU  - Seoane,RC
AU  - Li,JV
AU  - Marchesi,JR
AU  - Hart,AL
DO  - 10.1111/apt.16086
EP  - 1502
PY  - 2020///
SN  - 0269-2813
SP  - 1491
TI  - Multiomic features associated with mucosal healing and inflammation in paediatric Crohn's disease
T2  - Alimentary Pharmacology and Therapeutics
UR  - http://dx.doi.org/10.1111/apt.16086
UR  - https://www.ncbi.nlm.nih.gov/pubmed/32929796
UR  - https://onlinelibrary.wiley.com/doi/full/10.1111/apt.16086
UR  - http://hdl.handle.net/10044/1/82891
VL  - 52
ER  -
Download