Imperial College London
Portrait Picture

DrKieranO'Dea

Faculty of Medicine, Department of Surgery & Cancer

Senior Lecturer in Translational Critical Care
 
 
 
//

Contact

 

k.odea

 
 
//

Location

 

G3.43Chelsea and Westminster HospitalChelsea and Westminster Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Hua:2020:biolre/ioz188,
author = {Hua, R and Edey, LF and O'Dea, KP and Howe, L and Herbert, BR and Cheng, W and Zheng, X and MacIntyre, DA and Bennett, PR and Takata, M and Johnson, MR},
doi = {biolre/ioz188},
journal = {Biology of Reproduction},
pages = {445--455},
title = {CCR2 mediates the adverse effects of LPS in the pregnant mouse},
url = {http://dx.doi.org/10.1093/biolre/ioz188},
volume = {102},
year = {2020}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - In our earlier work, we found that intrauterine (i.u.) and intraperitoneal (i.p.) injection of LPS (10-μg serotype 0111:B4) induced preterm labor (PTL) with high pup mortality, marked systemic inflammatory response and hypotension. Here, we used both i.u. and i.p. LPS models in pregnant wild-type (wt) and CCR2 knockout (CCR2-/-) mice on E16 to investigate the role played by the CCL2/CCR2 system in the response to LPS. Basally, lower numbers of monocytes and macrophages and higher numbers of neutrophils were found in the myometrium, placenta, and blood of CCR2-/- vs. wt mice. After i.u. LPS, parturition occurred at 14 h in both groups of mice. At 7 h post-injection, 70% of wt pups were dead vs. 10% of CCR2-/- pups, but at delivery 100% of wt and 90% of CCR2-/- pups were dead. Myometrial and placental monocytes and macrophages were generally lower in CCR2-/- mice, but this was less consistent in the circulation, lung, and liver. At 7 h post-LPS, myometrial ERK activation was greater and JNK and p65 lower and the mRNA levels of chemokines were higher and of inflammatory cytokines lower in CCR2-/- vs. wt mice. Pup brain and placental inflammation were similar. Using the IP LPS model, we found that all measures of arterial pressure increased in CCR2-/- but declined in wt mice. These data suggest that the CCL2/CCR2 system plays a critical role in the cardiovascular response to LPS and contributes to pup death but does not influence the onset of inflammation-induced PTL.
AU  - Hua,R
AU  - Edey,LF
AU  - O'Dea,KP
AU  - Howe,L
AU  - Herbert,BR
AU  - Cheng,W
AU  - Zheng,X
AU  - MacIntyre,DA
AU  - Bennett,PR
AU  - Takata,M
AU  - Johnson,MR
DO  - biolre/ioz188
EP  - 455
PY  - 2020///
SN  - 0006-3363
SP  - 445
TI  - CCR2 mediates the adverse effects of LPS in the pregnant mouse
T2  - Biology of Reproduction
UR  - http://dx.doi.org/10.1093/biolre/ioz188
UR  - https://www.ncbi.nlm.nih.gov/pubmed/31599921
UR  - http://hdl.handle.net/10044/1/80172
VL  - 102
ER  -
Download