Imperial College London
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DrKieranO'Dea

Faculty of Medicine, Department of Surgery & Cancer

Senior Lecturer in Translational Critical Care
 
 
 
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Contact

 

k.odea

 
 
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Location

 

G3.43Chelsea and Westminster HospitalChelsea and Westminster Campus

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Summary

 

Publications

Citation

BibTex format

@article{Edey:2017:biolre/iox146,
author = {Edey, LF and Georgiou, H and O'Dea, KP and Mesiano, S and Herbert, BR and Lei, K and Hua, R and Markovic, D and Waddington, SN and MacIntyre, D and Bennett, P and Takata, M and Johnson, MR},
doi = {biolre/iox146},
journal = {Biology of Reproduction},
pages = {376--395},
title = {Progesterone, the maternal immune system and the onset of parturition in the mouse},
url = {http://dx.doi.org/10.1093/biolre/iox146},
volume = {98},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The role of progesterone (P4) in the regulation of the local (uterine) and systemic innate immune system, myometrial expression of connexin 43 (Cx-43) and cyclooxygenase 2 (COX-2) and the onset of parturition was examined in: 1) naïve mice delivering at term; 2) E16 mice treated with RU486 (P4-antagonist) to induce preterm parturition; and 3) in mice treated with P4 to prevent term parturition.In naïve mice, myometrial neutrophil and monocyte numbers peaked at E18 and declined with the onset of parturition. In contrast, circulating monocytes did not change and although neutrophils were increased with pregnancy, they did not change across gestation. The myometrial mRNA and protein levels of most chemokines/cytokines, Cx-43 and COX-2 increased with, but not before, parturition.With RU486-induced parturition, myometrial and systemic neutrophil numbers increased before and myometrial monocyte numbers increased with parturition only. Myometrial chemokine/cytokine mRNA abundance increased with parturition, but protein levels peaked earlier at between 4.5 and 9h post RU486. Cx-43, but not COX-2, mRNA expression and protein levels increased prior to the onset of parturition.In mice treated with P4, the gestation-linked increase in myometrial monocyte, but not neutrophil, numbers was prevented and expression of Cx-43 and COX-2 was reduced. On E20 of P4 supplementation, myometrial chemokine/cytokine and leukocyte numbers, but not Cx-43 and COX-2 expression, increased.These data show that during pregnancy P4 controls myometrial monocyte infiltration, cytokine and prolabour factor synthesis via mRNA dependent and independent mechanisms and, with prolonged P4 supplementation, P4 action is repressed resulting in increased myometrial inflammation.
AU  - Edey,LF
AU  - Georgiou,H
AU  - O'Dea,KP
AU  - Mesiano,S
AU  - Herbert,BR
AU  - Lei,K
AU  - Hua,R
AU  - Markovic,D
AU  - Waddington,SN
AU  - MacIntyre,D
AU  - Bennett,P
AU  - Takata,M
AU  - Johnson,MR
DO  - biolre/iox146
EP  - 395
PY  - 2017///
SN  - 1529-7268
SP  - 376
TI  - Progesterone, the maternal immune system and the onset of parturition in the mouse
T2  - Biology of Reproduction
UR  - http://dx.doi.org/10.1093/biolre/iox146
UR  - http://hdl.handle.net/10044/1/56976
VL  - 98
ER  -
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