Imperial College London
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Emeritus ProfessorKenMacLeod

Faculty of Medicine, National Heart & Lung Institute

Emeritus Professor of Cardiac Physiology
 
 
 
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Contact

 

+44 (0)20 7594 2734k.t.macleod

 
 
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Assistant

 

Miss Natasha Richmond +44 (0)20 7594 6457

 
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Location

 

336ICTEM buildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Thomas:2016:10.1016/j.bbrc.2016.12.169,
author = {Thomas, NM and Gray, R and Fry, CH and Desplantez, T and Peters, NS and Severs, NJ and Macleod, KT and Dupont, E},
doi = {10.1016/j.bbrc.2016.12.169},
journal = {Biochemical and Biophysical Research Communications},
pages = {191--196},
title = {Functional consequences of co-expressing connexin40 or connexin45 with connexin43 on intercellular electrical coupling},
url = {http://dx.doi.org/10.1016/j.bbrc.2016.12.169},
volume = {483},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The functional characteristics of the co-expression of connexin43, connexin40, and connexin45 proteins in human myocardium are thought to play an important role in governing normal propagation of the cardiac electrical impulse and in generating the myocardial substrate for some arrhythmias and conduction disturbances.A rat liver epithelial cell line, that endogenously expresses connexin43, was used to induce also expression of connexin40 or connexin45 after stable transfection using an inducible ecdysone system. Electrical coupling was estimated from measurement of the input resistance of transfected cells using an intracellular microelectrode to inject current and record changes to membrane potential.However, varied expression of the transfected connexin40 or connexin45 did not change electrical coupling, although connexin43/40 co-expression led to better coupling than connexin43/45 co-expression. Quantification of endogenous connexin43 expression, at both mRNA and protein levels, showed that it was altered in a manner dependent on the transfected connexin isotype.The data using rat liver epithelial cells indicate an increased electrical coupling upon expression of connexin40 and connexin43 but decreased coupling with connexin45 and connexin43 co-expression.
AU  - Thomas,NM
AU  - Gray,R
AU  - Fry,CH
AU  - Desplantez,T
AU  - Peters,NS
AU  - Severs,NJ
AU  - Macleod,KT
AU  - Dupont,E
DO  - 10.1016/j.bbrc.2016.12.169
EP  - 196
PY  - 2016///
SN  - 0006-291X
SP  - 191
TI  - Functional consequences of co-expressing connexin40 or connexin45 with connexin43 on intercellular electrical coupling
T2  - Biochemical and Biophysical Research Communications
UR  - http://dx.doi.org/10.1016/j.bbrc.2016.12.169
UR  - http://hdl.handle.net/10044/1/54551
VL  - 483
ER  -
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