Imperial College London

DrKonstantinosBeis

Faculty of Natural SciencesDepartment of Life Sciences

Senior Lecturer
 
 
 
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Contact

 

+44 (0)1235 567 809konstantinos.beis Website

 
 
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Location

 

1.18Diamond Light Source LtdHarwell Science and Innovation Campus

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Summary

 

Summary

MEMBRANE PROTEIN CRYSTALLOGRAPHY

Membrane proteins represent around 30% of the proteomes of most organisms and more than 40% of drug targets and yet few structures of these molecules have been solved by x-ray crystallography. Drug resistance of bacterial pathogens is a rising crisis. Bacterial membrane proteins are essential for resistance since they are involved in the export of the drugs from the cell. My group is interested in the structural and functional characterisation of multidrug membrane transporters by X-ray crystallography.

We are also interested to exploit novel antibacterials as treatments for bacterial infections.

Bacteria under nutrient starvation can produce antibacterial peptides that hijack outer and inner membrane proteins for internalisation and cell death. These peptides are also toxic to the producing bacteria which utilise ABC-transporters to provide them with immunity. We have solved the structure and fully characterised the ABC-transporter McjD. The protein is in an outward-occluded state and provides key mechanistic information on this family of transporters.

We also solved the Crystal structure of FhuA (blue and gold) in complex with MccJ25 (pink).structure of the outer membrane FhuA in complex with the antibacterial peptide MccJ25. The structure explains how antibacterial peptides can hijack the outer membrane and be internalised.

Publications

Journals

Bountra K, Hagelueken G, Choudhury HG, et al., 2017, Structural basis for antibacterial peptide self-immunity by the bacterial ABC transporter McjD., Embo J

Mehmood S, Corradi V, Choudhury HG, et al., 2016, Structural and Functional Basis for Lipid Synergy on the Activity of the Antibacterial Peptide ABC Transporter McjD, Journal of Biological Chemistry, Vol:291, ISSN:0021-9258, Pages:21656-21668

Axford D, Foadi J, Hu N-J, et al., 2015, Structure determination of an integral membrane protein at room temperature from crystals in situ, Acta Crystallographica Section D-structural Biology, Vol:71, ISSN:2059-7983, Pages:1228-1237

Beis K, 2015, Structural basis for the mechanism of ABC transporters, Biochemical Society Transactions, Vol:43, ISSN:0300-5127, Pages:889-893

Gu R-X, Corradi V, Singh G, et al., 2015, Conformational Changes of the ABC Transporter McjD Revealed by Molecular Dynamics Simulations, Biophysical Journal, Vol:108, ISSN:0006-3495, Pages:89A-89A

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