Imperial College London
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DrKonstantinosBeis

Faculty of Natural Sciences, Department of Life Sciences

Reader in Membrane Protein Structural Biology
 
 
 
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Contact

 

konstantinos.beis Website

 
 
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Location

 

1-12Diamond Light Source LtdHarwell Science and Innovation Campus

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Summary

 

Publications

Citation

BibTex format

@article{Husada:2018:10.15252/embj.2018100056,
author = {Husada, F and Bountra, K and Tasis, K and de, Boer M and Romano, M and Rebuffat, S and Beis, K and Cordes, T},
doi = {10.15252/embj.2018100056},
journal = {EMBO Journal},
title = {Conformational dynamics of the ABC transporter McjD seen by single-molecule FRET},
url = {http://dx.doi.org/10.15252/embj.2018100056},
volume = {37},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - ABC transporters utilize ATP for export processes to provide cellular resistance against toxins, antibiotics, and harmful metabolites in eukaryotes and prokaryotes. Based on static structure snapshots, it is believed that they use an alternating access mechanism, which couples conformational changes to ATP binding (outwardopen conformation) and hydrolysis (inwardopen) for unidirectional transport driven by ATP. Here, we analyzed the conformational states and dynamics of the antibacterial peptide exporter McjD from Escherichia coli using singlemolecule Förster resonance energy transfer (smFRET). For the first time, we established smFRET for an ABC exporter in a nativelike lipid environment and directly monitor conformational dynamics in both the transmembrane (TMD) and nucleotidebinding domains (NBD). With this, we unravel the ligand dependences that drive conformational changes in both domains. Furthermore, we observe intrinsic conformational dynamics in the absence of ATP and ligand in the NBDs. ATP binding and hydrolysis on the other hand can be observed via NBD conformational dynamics. We believe that the progress made here in combination with future studies will facilitate full understanding of ABC transport cycles.
AU  - Husada,F
AU  - Bountra,K
AU  - Tasis,K
AU  - de,Boer M
AU  - Romano,M
AU  - Rebuffat,S
AU  - Beis,K
AU  - Cordes,T
DO  - 10.15252/embj.2018100056
PY  - 2018///
SN  - 0261-4189
TI  - Conformational dynamics of the ABC transporter McjD seen by single-molecule FRET
T2  - EMBO Journal
UR  - http://dx.doi.org/10.15252/embj.2018100056
UR  - http://hdl.handle.net/10044/1/64373
VL  - 37
ER  -
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