Imperial College London
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DrKonstantinosBeis

Faculty of Natural Sciences, Department of Life Sciences

Reader in Membrane Protein Structural Biology
 
 
 
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Contact

 

konstantinos.beis Website

 
 
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Location

 

1-12Diamond Light Source LtdHarwell Science and Innovation Campus

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Summary

 

Publications

Citation

BibTex format

@article{Wang:2019:10.1038/s42003-019-0317-6,
author = {Wang, L and Bateman, B and Zanetti-Domingues, LC and Moores, AN and Astbury, S and Spindloe, C and Darrow, MC and Romano, M and Needham, SR and Beis, K and Rolfe, DJ and Clarke, DT and Martin-Fernandez, ML},
doi = {10.1038/s42003-019-0317-6},
journal = {Communications Biology},
title = {Solid immersion microscopy images cells under cryogenic conditions with 12 nm resolution},
url = {http://dx.doi.org/10.1038/s42003-019-0317-6},
volume = {2},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Super-resolution fluorescence microscopy plays a crucial role in our understanding of cell structure and function by reporting cellular ultrastructure with 20–30 nm resolution. However, this resolution is insufficient to image macro-molecular machinery at work. A path to improve resolution is to image under cryogenic conditions. This substantially increases the brightness of most fluorophores and preserves native ultrastructure much better than chemical fixation. Cryogenic conditions are, however, underutilised because of the lack of compatible high numerical aperture objectives. Here, using a low-cost super-hemispherical solid immersion lens (superSIL) and a basic set-up we achieve 12 nm resolution under cryogenic conditions, to our knowledge the best yet attained in cells using simple set-ups and/or commercial systems. By also allowing multicolour imaging, and by paving the way to total-internal-reflection fluorescence imaging of mammalian cells under cryogenic conditions, superSIL microscopy opens a straightforward route to achieve unmatched resolution on bacterial and mammalian cell samples.
AU  - Wang,L
AU  - Bateman,B
AU  - Zanetti-Domingues,LC
AU  - Moores,AN
AU  - Astbury,S
AU  - Spindloe,C
AU  - Darrow,MC
AU  - Romano,M
AU  - Needham,SR
AU  - Beis,K
AU  - Rolfe,DJ
AU  - Clarke,DT
AU  - Martin-Fernandez,ML
DO  - 10.1038/s42003-019-0317-6
PY  - 2019///
SN  - 2399-3642
TI  - Solid immersion microscopy images cells under cryogenic conditions with 12 nm resolution
T2  - Communications Biology
UR  - http://dx.doi.org/10.1038/s42003-019-0317-6
UR  - http://hdl.handle.net/10044/1/67894
VL  - 2
ER  -
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