Imperial College London
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DrLeandroCastellano

Faculty of Medicine, Department of Surgery & Cancer

Visiting Reader
 
 
 
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Contact

 

+44 (0)20 7594 2822l.castellano Website

 
 
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Location

 

ICTEM buildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Jacob:2015:10.1016/j.canlet.2015.12.026,
author = {Jacob, J and Favicchio, R and Karimian, N and Mehrabi, M and Harding, V and Castellano, L and Stebbing, J and Giamas, G},
doi = {10.1016/j.canlet.2015.12.026},
journal = {Cancer Letters},
pages = {137--146},
title = {LMTK3 escapes tumour suppressor miRNAs via sequestration of DDX5.},
url = {http://dx.doi.org/10.1016/j.canlet.2015.12.026},
volume = {372},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Lemur tyrosine kinase-3 (LMTK3) plays an important role in cancer progression and is associated with breast, lung, gastric and colorectal cancer. MicroRNAs (miRNAs) are small endogenous non-coding RNAs that typically repress target genes at post-transcriptional level and have an important role in tumorigenesis. By performing a miRNA expression profile, we identified a subset of miRNAs modulated by LMTK3. We show that LMTK3 induces miR-34a, miR-196-a2 and miR-182 levels interacting with DEAD-box RNA helicase p68 (DDX5). LMTK3 binds via DDX5 to the pri-miRNA of these three mature miRNAs, thereby sequestrating them from further processing. Ectopic expression of miR-34a and miR-182 in LMTK3-overexpressing cell lines (MCF7-LMTK3 and MDA-MB-231-LMTK3) inhibits breast cancer proliferation, invasion and migration. Interestingly, miR-34a and miR-182 directly bind to the 3'UTR of LMTK3 mRNA and consequently inhibit both its stability and translation, acting as tumour suppressor-like miRNAs. In aggregate, we show that LMTK3 is involved in miRNA biogenesis through modulation of the Microprocessor complex, inducing miRNAs that target LMTK3 itself.
AU  - Jacob,J
AU  - Favicchio,R
AU  - Karimian,N
AU  - Mehrabi,M
AU  - Harding,V
AU  - Castellano,L
AU  - Stebbing,J
AU  - Giamas,G
DO  - 10.1016/j.canlet.2015.12.026
EP  - 146
PY  - 2015///
SN  - 1872-7980
SP  - 137
TI  - LMTK3 escapes tumour suppressor miRNAs via sequestration of DDX5.
T2  - Cancer Letters
UR  - http://dx.doi.org/10.1016/j.canlet.2015.12.026
VL  - 372
ER  -
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