Imperial College London
Alternate

Dr Lata Govada

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Research Associate
 
 
 
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Contact

 

+44 (0)20 7594 3037l.govada

 
 
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Location

 

Open Plan no 12Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Gillis:2021:10.1038/s41598-021-81251-2,
author = {Gillis, RB and Solomon, HV and Govada, L and Oldham, NJ and Dinu, V and Jiwani, SI and Gyasi-Antwi, P and Coffey, F and Meal, A and Morgan, PS and Harding, SE and Helliwell, JR and Chayen, NE and Adams, GG},
doi = {10.1038/s41598-021-81251-2},
journal = {Scientific Reports},
title = {Analysis of insulin glulisine at the molecular level by X-ray crystallography and biophysical techniques},
url = {http://dx.doi.org/10.1038/s41598-021-81251-2},
volume = {11},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - This study concerns glulisine, a rapid-acting insulin analogue that plays a fundamental role in diabetes management. We have applied a combination of methods namely X-ray crystallography, and biophysical characterisation to provide a detailed insight into the structure and function of glulisine. X-ray data provided structural information to a resolution of 1.26 Å. Crystals belonged to the H3 space group with hexagonal (centred trigonal) cell dimensions a = b = 82.44 and c = 33.65 Å with two molecules in the asymmetric unit. A unique position of D21Glu, not present in other fast-acting analogues, pointing inwards rather than to the outside surface was observed. This reduces interactions with neighbouring molecules thereby increasing preference of the dimer form. Sedimentation velocity/equilibrium studies revealed a trinary system of dimers and hexamers/dihexamers in dynamic equilibrium. This new information may lead to better understanding of the pharmacokinetic and pharmacodynamic behaviour of glulisine which might aid in improving formulation regarding its fast-acting role and reducing side effects of this drug.
AU  - Gillis,RB
AU  - Solomon,HV
AU  - Govada,L
AU  - Oldham,NJ
AU  - Dinu,V
AU  - Jiwani,SI
AU  - Gyasi-Antwi,P
AU  - Coffey,F
AU  - Meal,A
AU  - Morgan,PS
AU  - Harding,SE
AU  - Helliwell,JR
AU  - Chayen,NE
AU  - Adams,GG
DO  - 10.1038/s41598-021-81251-2
PY  - 2021///
SN  - 2045-2322
TI  - Analysis of insulin glulisine at the molecular level by X-ray crystallography and biophysical techniques
T2  - Scientific Reports
UR  - http://dx.doi.org/10.1038/s41598-021-81251-2
UR  - https://www.ncbi.nlm.nih.gov/pubmed/33462295
UR  - http://hdl.handle.net/10044/1/86225
VL  - 11
ER  -
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