Lisete M. Silva obtained her BSc and MSc degrees in Chemistry at the Faculty of Sciences, Porto University (Portugal). Her PhD degree in Biochemistry at University of Aveiro (Portugal) was under the Supervision of Professor Manuel A. Coimbra with co-supervisors Dr. Angelina S. Palma (NOVA University of Lisbon, Portugal) and Dr. José A. Ferreira (Portuguese Institute of Oncology Porto).
Lisete’s first experience in the Glycosciences Laboratory, directed by Professor Ten Feizi at Imperial College London, was in 2014 as a visiting student during her PhD studies, funded by the Portuguese Foundation for Science and Technology. Her work was on structural analysis of lipopolysaccharides (LPSs) isolated from clinical Helicobacter pylori strains by mass spectrometry: GC-MS and ESI-MS/MSn, and development of microarrays of the LPSs for interaction studies with carbohydrate recognising proteins: plant and animal lectins and proteins of the immune system. The LPS microarrays are also being used for serological studies aimed at distinguishing H. pylori-infected and uninfected subjects.
Lisete started her Research Associate post in the Glycosciences Laboratory in March 2015; and in November 2015 she was named Deputy Microarray Project Leader with responsibility for (a) running and maintaining the group’s microarray equipment and (b) performing glycan array analyses of proteins received from national and international collaborators. She carried out investigations of different liposomal formulations for lipid-linked glycan probes in neoglycolipid (NGL)-based microarrays with respect to the binding signals they elicit in microarray analyses. These studies led to the construction of a new version of a broad spectrum microarray of 896 glycan probes for the Wellcome-Trust supported Carbohydrate Microarray Facility to apply in high throughput analyses of proteins of biological and medical impact.
As an enthusiastic and skilled researcher, Lisete is interested to further explore pathogen-host interactions that trigger disease processes, and establish industrial contacts for translational research collaborations in Biomedicine.
et al., 2020, Mannan detecting C-type lectin receptor probes recognise immune epitopes with diverse chemical, spatial and phylogenetic heterogeneity in fungal cell walls, Plos Pathogens, Vol:16, ISSN:1553-7366
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et al., 2019, Sulfated glycosaminoglycans as viral decoy receptors for human adenovirus type 37, Viruses, Vol:11, ISSN:1999-4915
et al., 2019, Single human B cell-derived monoclonal anti-Candida antibodies enhance phagocytosis and protect against disseminated candidiasis (vol 9, 5288, 2018), Nature Communications, Vol:10, ISSN:2041-1723
et al., 2018, Single human B cell-derived monoclonal anti-Candida antibodies enhance phagocytosis and protect against disseminated candidiasis, Nature Communications, Vol:9, ISSN:2041-1723