Imperial College London


Faculty of MedicineDepartment of Surgery & Cancer

Chair in Cancer Adaptation and Evolution



+44 (0)20 7594 2808l.magnani CV




137ICTEM buildingHammersmith Campus






BibTex format

author = {Zhang, Y and Takahashi, Y and Hong, SP and Liu, F and Bednarska, J and Goff, P and Novak, P and Shevchuk, A and Gopal, S and Barozzi, I and Magnani, L and Sakai, H and Suguru, Y and Fujii, T and Gorelkin, P and Majouga, A and Weiss, D and Edwards, C and Ivanov, A and Klenerman, D and Sviderskaya, E and Edel, J and Korchev, Y},
doi = {10.1038/s41467-019-13535-1},
journal = {Nature Communications},
title = {High-resolution label-free 3D mapping of extracellular pH of single living cells},
url = {},
volume = {10},
year = {2019}

RIS format (EndNote, RefMan)

AB - Dynamic mapping of extracellular pH (pHe) at the single-cell level is critical for understanding the role of H+ in cellular and subcellular processes, with particular importance in cancer. While several pHe sensing techniques have been developed, accessing this information at the single-cell level requires improvement in sensitivity, spatial and temporal resolution. We report on a zwitterionic label-free pH nanoprobe that addresses these long-standing challenges. The probe has a sensitivity >0.01 units, 2 ms response time, and 50 nm spatial resolution. The technology was incorporated into a double-barrel nanoprobe integrating pH sensing with feedback-controlled distance sensing via Scanning Ion Conductance Microscopy. This allows for the simultaneous 3D topographical imaging and pHe monitoring of living cancer cells. These classes of nanoprobes were used for real-time high spatiotemporal resolution pHe mapping at the subcellular level and revealed tumour heterogeneity of the peri-cellular environments of melanoma and breast cancer cells.
AU - Zhang,Y
AU - Takahashi,Y
AU - Hong,SP
AU - Liu,F
AU - Bednarska,J
AU - Goff,P
AU - Novak,P
AU - Shevchuk,A
AU - Gopal,S
AU - Barozzi,I
AU - Magnani,L
AU - Sakai,H
AU - Suguru,Y
AU - Fujii,T
AU - Gorelkin,P
AU - Majouga,A
AU - Weiss,D
AU - Edwards,C
AU - Ivanov,A
AU - Klenerman,D
AU - Sviderskaya,E
AU - Edel,J
AU - Korchev,Y
DO - 10.1038/s41467-019-13535-1
PY - 2019///
SN - 2041-1723
TI - High-resolution label-free 3D mapping of extracellular pH of single living cells
T2 - Nature Communications
UR -
UR -
VL - 10
ER -