Imperial College London


Faculty of MedicineDepartment of Surgery & Cancer

Chair in Cancer Adaptation and Evolution



+44 (0)20 7594 2808l.magnani CV




137ICTEM buildingHammersmith Campus






BibTex format

author = {Zhang, Y and Clausmeyer, J and Babakinejad, B and López, Córdoba A and Ali, T and Shevchuk, A and Takahashi, Y and Novak, P and Edwards, C and Lab, M and Gopal, S and Chiappini, C and Anand, U and Magnani, L and Coombes, RC and Gorelik, J and Matsue, T and Schuhmann, W and Klenerman, D and Sviderskaya, EV and Korchev, Y},
doi = {10.1021/acsnano.5b05211},
journal = {ACS Nano},
pages = {3214--3221},
title = {Spearhead Nanometric Field-Effect Transistor Sensors for Single-Cell Analysis.},
url = {},
volume = {10},
year = {2016}

RIS format (EndNote, RefMan)

AB - Nanometric field-effect-transistor (FET) sensors are made on the tip of spear-shaped dual carbon nanoelectrodes derived from carbon deposition inside double-barrel nanopipettes. The easy fabrication route allows deposition of semiconductors or conducting polymers to comprise the transistor channel. A channel from electrodeposited poly pyrrole (PPy) exhibits high sensitivity toward pH changes. This property is exploited by immobilizing hexokinase on PPy nano-FETs to give rise to a selective ATP biosensor. Extracellular pH and ATP gradients are key biochemical constituents in the microenvironment of living cells; we monitor their real-time changes in relation to cancer cells and cardiomyocytes. The highly localized detection is possible because of the high aspect ratio and the spear-like design of the nano-FET probes. The accurately positioned nano-FET sensors can detect concentration gradients in three-dimensional space, identify biochemical properties of a single living cell, and after cell membrane penetration perform intracellular measurements.
AU - Zhang,Y
AU - Clausmeyer,J
AU - Babakinejad,B
AU - López,Córdoba A
AU - Ali,T
AU - Shevchuk,A
AU - Takahashi,Y
AU - Novak,P
AU - Edwards,C
AU - Lab,M
AU - Gopal,S
AU - Chiappini,C
AU - Anand,U
AU - Magnani,L
AU - Coombes,RC
AU - Gorelik,J
AU - Matsue,T
AU - Schuhmann,W
AU - Klenerman,D
AU - Sviderskaya,EV
AU - Korchev,Y
DO - 10.1021/acsnano.5b05211
EP - 3221
PY - 2016///
SN - 1936-086X
SP - 3214
TI - Spearhead Nanometric Field-Effect Transistor Sensors for Single-Cell Analysis.
T2 - ACS Nano
UR -
UR -
VL - 10
ER -