Imperial College London

DrLucaMagnani

Faculty of MedicineDepartment of Surgery & Cancer

Principal Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 2808l.magnani CV

 
 
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Location

 

140ICTEM buildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Menyhárt:2016:10.1016/j.bbcan.2016.10.002,
author = {Menyhárt, O and Harami-Papp, H and Sukumar, S and Schäfer, R and Magnani, L and de, Barrios O and Gyrffy, B},
doi = {10.1016/j.bbcan.2016.10.002},
journal = {Biochimica et Biophysica Acta-Reviews on Cancer},
pages = {300--319},
title = {Guidelines for the selection of functional assays to evaluate the hallmarks of cancer},
url = {http://dx.doi.org/10.1016/j.bbcan.2016.10.002},
volume = {1866},
year = {2016}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - The hallmarks of cancer capture the most essential phenotypic characteristics of malignant transformation and progression. Although numerous factors involved in this multi-step process are still unknown to date, an ever-increasing number of mutated/altered candidate genes are being identified within large-scale cancer genomic projects. Therefore, investigators need to be aware of available and appropriate techniques capable of determining characteristic features of each hallmark.We review the methods tailored to experimental cancer researchers to evaluate cell proliferation, programmed cell death, replicative immortality, induction of angiogenesis, invasion and metastasis, genome instability, and reprogramming of energy metabolism. Selecting the ideal method is based on the investigator's goals, available equipment and also on financial constraints. Multiplexing strategies enable a more in-depth data collection from a single experiment — obtaining several results from a single procedure reduces variability and saves time and relative cost, leading to more robust conclusions compared to a single end point measurement. Each hallmark possesses characteristics that can be analyzed by immunoblot, RT-PCR, immunocytochemistry, immunoprecipitation, RNA microarray or RNA-seq. In general, flow cytometry, fluorescence microscopy, and multiwell readers are extremely versatile tools and, with proper sample preparation, allow the detection of a vast number of hallmark features. Finally, we also provide a list of hallmark-specific genes to be measured in transcriptome-level studies.Although our list is not exhaustive, we provide a snapshot of the most widely used methods, with an emphasis on methods enabling the simultaneous evaluation of multiple hallmark features.
AU - Menyhárt,O
AU - Harami-Papp,H
AU - Sukumar,S
AU - Schäfer,R
AU - Magnani,L
AU - de,Barrios O
AU - Gyrffy,B
DO - 10.1016/j.bbcan.2016.10.002
EP - 319
PY - 2016///
SN - 0304-419X
SP - 300
TI - Guidelines for the selection of functional assays to evaluate the hallmarks of cancer
T2 - Biochimica et Biophysica Acta-Reviews on Cancer
UR - http://dx.doi.org/10.1016/j.bbcan.2016.10.002
UR - http://www.ncbi.nlm.nih.gov/pubmed/27742530
UR - http://hdl.handle.net/10044/1/42912
VL - 1866
ER -