Imperial College London

DrLucaMagnani

Faculty of MedicineDepartment of Surgery & Cancer

Principal Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 2808l.magnani CV

 
 
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Location

 

140ICTEM buildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Noberini:2018:nar/gky224,
author = {Noberini, R and Osti, D and Miccolo, C and Richichi, C and Lupia, M and Corleone, G and Hong, S-P and Colombo, P and Pollo, B and Fornasari, L and Pruneri, G and Magnani, L and Cavallaro, U and Chiocca, S and Minucci, S and Pelicci, G and Bonaldi, T},
doi = {nar/gky224},
journal = {Nucleic Acids Research},
pages = {3817--3832},
title = {Extensive and systematic rewiring of histone post-translational modifications in cancer model systems},
url = {http://dx.doi.org/10.1093/nar/gky224},
volume = {46},
year = {2018}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Histone post-translational modifications (PTMs) generate a complex combinatorial code that regulates gene expression and nuclear functions, and whose deregulation has been documented in different types of cancers. Therefore, the availability of relevant culture models that can be manipulated and that retain the epigenetic features of the tissue of origin is absolutely crucial for studying the epigenetic mechanisms underlying cancer and testing epigenetic drugs. In this study, we took advantage of quantitative mass spectrometry to comprehensively profile histone PTMs in patient tumor tissues, primary cultures and cell lines from three representative tumor models, breast cancer, glioblastoma and ovarian cancer, revealing an extensive and systematic rewiring of histone marks in cell culture conditions, which includes a decrease of H3K27me2/me3, H3K79me1/me2 and H3K9ac/K14ac, and an increase of H3K36me1/me2. While some changes occur in short-term primary cultures, most of them are instead time-dependent and appear only in long-term cultures. Remarkably, such changes mostly revert in cell line- and primary cell-derived in vivo xenograft models. Taken together, these results support the use of xenografts as the most representative models of in vivo epigenetic processes, suggesting caution when using cultured cells, in particular cell lines and long-term primary cultures, for epigenetic investigations.
AU - Noberini,R
AU - Osti,D
AU - Miccolo,C
AU - Richichi,C
AU - Lupia,M
AU - Corleone,G
AU - Hong,S-P
AU - Colombo,P
AU - Pollo,B
AU - Fornasari,L
AU - Pruneri,G
AU - Magnani,L
AU - Cavallaro,U
AU - Chiocca,S
AU - Minucci,S
AU - Pelicci,G
AU - Bonaldi,T
DO - nar/gky224
EP - 3832
PY - 2018///
SN - 0305-1048
SP - 3817
TI - Extensive and systematic rewiring of histone post-translational modifications in cancer model systems
T2 - Nucleic Acids Research
UR - http://dx.doi.org/10.1093/nar/gky224
UR - https://www.ncbi.nlm.nih.gov/pubmed/29618087
UR - http://hdl.handle.net/10044/1/58961
VL - 46
ER -