Imperial College London

DrLucaMagnani

Faculty of MedicineDepartment of Surgery & Cancer

Senior Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 2808l.magnani CV

 
 
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Location

 

140ICTEM buildingHammersmith Campus

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Summary

 

Publications

Publication Type
Year
to

56 results found

Harrod A, Fulton J, Nguyen VTM, Periyasamy M, Ramos-Garcia L, Lai C-F, Metodieva G, de Giorgio A, Williams RL, Santos DB, Gomez PJ, Lin M-L, Metodiev MV, Stebbing J, Castellano L, Magnani L, Coombes RC, Buluwela L, Ali Set al., 2017, Genomic modelling of the ESR1 Y537S mutation for evaluating function and new therapeutic approaches for metastatic breast cancer, ONCOGENE, Vol: 36, Pages: 2286-2296, ISSN: 0950-9232

JOURNAL ARTICLE

Magnani L, Frige G, Gadaleta RM, Corleone G, Fabris S, Kempe H, Verschure PJ, Barozzi I, Vircillo V, Hong S-P, Perone Y, Saini M, Trumpp A, Viale G, Neri A, Ali S, Colleoni MA, Pruneri G, Minucci Set al., 2017, Acquired CYP19A1 amplification is an early specific mechanism of aromatase inhibitor resistance in ER alpha metastatic breast cancer, NATURE GENETICS, Vol: 49, Pages: 444-450, ISSN: 1061-4036

JOURNAL ARTICLE

Magnani L, Frigè G, Gadaleta RM, Corleone G, Fabris S, Kempe MH, Vershure PJ, Barozzi I, Vircillo V, Hong S-P, Perone Y, Saini M, Trumpp A, Viale G, Neri A, Ali S, Colleoni MA, Pruneri G, Minucci Set al., 2017, Corrigendum: Acquired CYP19A1 amplification is an early specific mechanism of aromatase inhibitor resistance in ERα metastatic breast cancer., Nat Genet, Vol: 49

JOURNAL ARTICLE

Bhat-Nakshatri P, Goswami CP, Badve S, Magnani L, Lupien M, Nakshatri Het al., 2016, Molecular Insights of Pathways Resulting from Two Common PIK3CA Mutations in Breast Cancer, CANCER RESEARCH, Vol: 76, Pages: 3989-4001, ISSN: 0008-5472

JOURNAL ARTICLE

Chen X, Jung J-G, Shajahan-Haq AN, Clarke R, Shih I-M, Wang Y, Magnani L, Wang T-L, Xuan Jet al., 2016, ChIP-BIT: Bayesian inference of target genes using a novel joint probabilistic model of ChIP-seq profiles, NUCLEIC ACIDS RESEARCH, Vol: 44, ISSN: 0305-1048

JOURNAL ARTICLE

Hong SP, Lombardo Y, Corleone G, Coombes CR, Magnani Let al., 2016, Epigenetic plasticity identifies seed breast cancer cells driving drug resistance and metastatic progression, UK Breast Cancer Research Symposium, Publisher: SPRINGER, Pages: 191-191, ISSN: 0167-6806

CONFERENCE PAPER

Menyhart O, Harami-Papp H, Sukumar S, Schaefer R, Magnani L, de Barrios O, Gyorffy Bet al., 2016, Guidelines for the selection of functional assays to evaluate the hallmarks of cancer, BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER, Vol: 1866, Pages: 300-319, ISSN: 0304-419X

JOURNAL ARTICLE

Patel H, Abduljabbar R, Lai C-F, Periyasamy M, Harrod A, Gemma C, Steel JH, Patel N, Busonero C, Jerjees D, Remenyi J, Smith S, Gomm JJ, Magnani L, Gyorffy B, Jones LJ, Fuller-Pace F, Shousha S, Buluwela L, Rakha EA, Ellis IO, Coombes RC, Ali Set al., 2016, Expression of CDK7, Cyclin H, and MAT1 Is Elevated in Breast Cancer and Is Prognostic in Estrogen Receptor-Positive Breast Cancer, CLINICAL CANCER RESEARCH, Vol: 22, Pages: 5929-5938, ISSN: 1078-0432

JOURNAL ARTICLE

Patel H, Abduljabbar R, Lai C-F, Periyasamy M, Harrod A, Gemma C, Steel JH, Patel N, Busonero C, Jerjees D, Remenyi J, Smith S, Gomm JJ, Magnani L, Győrffy B, Jones LJ, Fuller-Pace F, Shousha S, Buluwela L, Rakha EA, Ellis IO, Coombes RC, Ali Set al., 2016, Expression of CDK7, Cyclin H, and MAT1 Is Elevated in Breast Cancer and Is Prognostic in Estrogen Receptor-Positive Breast Cancer., Clin Cancer Res, Vol: 22, Pages: 5929-5938, ISSN: 1078-0432

PURPOSE: CDK-activating kinase (CAK) is required for the regulation of the cell cycle and is a trimeric complex consisting of cyclin-dependent kinase 7 (CDK7), Cyclin H, and the accessory protein, MAT1. CDK7 also plays a critical role in regulating transcription, primarily by phosphorylating RNA polymerase II, as well as transcription factors such as estrogen receptor-α (ER). Deregulation of cell cycle and transcriptional control are general features of tumor cells, highlighting the potential for the use of CDK7 inhibitors as novel cancer therapeutics. EXPERIMENTAL DESIGN: mRNA and protein expression of CDK7 and its essential cofactors cyclin H and MAT1 were evaluated in breast cancer samples to determine if their levels are altered in cancer. Immunohistochemical staining of >900 breast cancers was used to determine the association with clinicopathologic features and patient outcome. RESULTS: We show that expressions of CDK7, cyclin H, and MAT1 are all closely linked at the mRNA and protein level, and their expression is elevated in breast cancer compared with the normal breast tissue. Intriguingly, CDK7 expression was inversely proportional to tumor grade and size, and outcome analysis showed an association between CAK levels and better outcome. Moreover, CDK7 expression was positively associated with ER expression and in particular with phosphorylation of ER at serine 118, a site important for ER transcriptional activity. CONCLUSIONS: Expressions of components of the CAK complex, CDK7, MAT1, and Cyclin H are elevated in breast cancer and correlate with ER. Like ER, CDK7 expression is inversely proportional to poor prognostic factors and survival. Clin Cancer Res; 22(23); 5929-38. ©2016 AACR.

JOURNAL ARTICLE

Perone Y, Magnani L, 2016, Going off the grid: ER alpha breast cancer beyond estradiol, JOURNAL OF MOLECULAR ENDOCRINOLOGY, Vol: 57, Pages: F1-F5, ISSN: 0952-5041

JOURNAL ARTICLE

Zhang Y, Clausmeyer J, Babakinejad B, Cordoba AL, Ali T, Shevchuk A, Takahashi Y, Novak P, Edwards C, Lab M, Gopal S, Chiappini C, Anand U, Magnani L, Coombes RC, Gorelik J, Matsue T, Schuhmann W, Klenerman D, Sviderskaya EV, Korchev Yet al., 2016, Spearhead Nanometric Field-Effect Transistor Sensors for Single-Cell Analysis, ACS NANO, Vol: 10, Pages: 3214-3221, ISSN: 1936-0851

JOURNAL ARTICLE

Faronato M, Nguyen VTM, Patten DK, Lombardo Y, Steel JH, Patel N, Woodley L, Shousha S, Pruneri G, Coombes RC, Magnani Let al., 2015, DMXL2 drives epithelial to mesenchymal transition in hormonal therapy resistant breast cancer through notch hyper-activation, ONCOTARGET, Vol: 6, Pages: 22467-22479, ISSN: 1949-2553

JOURNAL ARTICLE

Magnani L, Patten DK, Nguyen VTM, Hong S-P, Steel JH, Patel N, Lombardo Y, Faronato M, Gomes AR, Woodley L, Page K, Guttery D, Primrose L, Garcia DF, Shaw J, Viola P, Green A, Nolan C, Ellis IO, Rakha EA, Shousha S, Lam EW-F, Gyorffy B, Lupien M, Coombes RCet al., 2015, The pioneer factor PBX1 is a novel driver of metastatic progression in ER'-positive breast cancer, ONCOTARGET, Vol: 6, Pages: 21878-21891, ISSN: 1949-2553

JOURNAL ARTICLE

Nakshatri H, Goswami C, Badve S, Magnani L, Lupien M, Bhat-Nakshatri Pet al., 2015, Divergent activation of AKT1 and AKT2 isoforms downstream of PI3K mutation impacts response of breast cancer cells to estradiol and PI3K inhibitors, 37th Annual CTRC-AACR San Antonio Breast Cancer Symposium, Publisher: AMER ASSOC CANCER RESEARCH, ISSN: 0008-5472

CONFERENCE PAPER

Nguyen VTM, Barozzi I, Faronato M, Lombardo Y, Steel JH, Patel N, Darbre P, Castellano L, Gyorffy B, Woodley L, Meira A, Patten DK, Vircillo V, Periyasamy M, Ali S, Frige G, Minucci S, Coombes RC, Magnani Let al., 2015, Differential epigenetic reprogramming in response to specific endocrine therapies promotes cholesterol biosynthesis and cellular invasion, NATURE COMMUNICATIONS, Vol: 6, ISSN: 2041-1723

JOURNAL ARTICLE

Okamoto OK, Matheu A, Magnani L, 2015, Stem Cells in Translational Cancer Research, STEM CELLS INTERNATIONAL, ISSN: 1687-966X

JOURNAL ARTICLE

Periyasamy M, Patel H, Lai C-F, Nguyen VTM, Nevedomskaya E, Harrod A, Russell R, Remenyi J, Ochocka AM, Thomas RS, Fuller-Pace F, Gyorffy B, Caldas C, Navaratnam N, Carroll JS, Zwart W, Coombes RC, Magnani L, Buluwela L, Ali Set al., 2015, APOBEC3B-Mediated Cytidine Deamination Is Required for Estrogen Receptor Action in Breast Cancer, CELL REPORTS, Vol: 13, Pages: 108-121, ISSN: 2211-1247

JOURNAL ARTICLE

Varghese V, Magnani L, Harada N, Eric LW, Kenny Let al., 2015, Inhibition of FOXM1 by thiostrepton increases sensitivity to 5-fluorouracil (5-FU) by downregulating thymidylate synthase (TS) in colorectal cancer, AACR Precision Medicine Conference on Drug Sensitivity and Resistance - Improving Cancer Therapy, Publisher: AMER ASSOC CANCER RESEARCH, ISSN: 1078-0432

CONFERENCE PAPER

Xu Y, Zhang H, Van TMN, Angelopoulos N, Nunes J, Reid A, Buluwela L, Magnani L, Stebbing J, Giamas Get al., 2015, LMTK3 represses tumor suppressor-like genes through chromatin remodeling in breast cancer, Cell Reports, Vol: 12, Pages: 837-849, ISSN: 2211-1247

LMTK3 is an oncogenic receptor tyrosine kinase (RTK) implicated in various types of cancer, including breast, lung, gastric, and colorectal cancer. It is localized in different cellular compartments, but its nuclear function has not been investigated so far. We mapped LMTK3 binding across the genome using ChIP-seq and found that LMTK3 binding events are correlated with repressive chromatin markers. We further identified KRAB-associated protein 1 (KAP1) as a binding partner of LMTK3. The LMTK3/KAP1 interaction is stabilized by PP1α, which suppresses KAP1 phosphorylation specifically at LMTK3-associated chromatin regions, inducing chromatin condensation and resulting in transcriptional repression of LMTK3-bound tumor suppressor-like genes. Furthermore, LMTK3 functions at distal regions in tethering the chromatin to the nuclear periphery, resulting in H3K9me3 modification and gene silencing. In summary, we propose a model where a scaffolding function of nuclear LMTK3 promotes cancer progression through chromatin remodeling.

JOURNAL ARTICLE

Bianco S, Brunelle M, Jangal M, Magnani L, Gevry Net al., 2014, LRH-1 Governs Vital Transcriptional Programs in Endocrine-Sensitive and -Resistant Breast Cancer Cells, CANCER RESEARCH, Vol: 74, Pages: 2015-2025, ISSN: 0008-5472

JOURNAL ARTICLE

Brown R, Curry E, Magnani L, Wilhelm-Benartzi CS, Borley Jet al., 2014, Poised epigenetic states and acquired drug resistance in cancer, NATURE REVIEWS CANCER, Vol: 14, Pages: 747-753, ISSN: 1474-175X

JOURNAL ARTICLE

Gadaleta RM, Magnani L, 2014, Nuclear receptors and chromatin: an inducible couple, JOURNAL OF MOLECULAR ENDOCRINOLOGY, Vol: 52, Pages: R137-R149, ISSN: 0952-5041

JOURNAL ARTICLE

Jangal M, Couture J-P, Bianco S, Magnani L, Mohammed H, Gevry Net al., 2014, The transcriptional co-repressor TLE3 suppresses basal signaling on a subset of estrogen receptor alpha target genes, NUCLEIC ACIDS RESEARCH, Vol: 42, Pages: 11339-11348, ISSN: 0305-1048

JOURNAL ARTICLE

Lombardo Y, Faronato M, Filipovic A, Vircillo V, Magnani L, Coombes RCet al., 2014, Nicastrin and Notch4 drive endocrine therapy resistance and epithelial to mesenchymal transition in MCF7 breast cancer cells, BREAST CANCER RESEARCH, Vol: 16, ISSN: 1465-542X

JOURNAL ARTICLE

Magnani L, 2014, Epigenetic engineering and the art of epigenetic manipulation, GENOME BIOLOGY, Vol: 15, ISSN: 1465-6906

JOURNAL ARTICLE

Magnani L, Lupien M, 2014, Chromatin and epigenetic determinants of estrogen receptor alpha (ESR1) signaling, MOLECULAR AND CELLULAR ENDOCRINOLOGY, Vol: 382, Pages: 633-641, ISSN: 0303-7207

JOURNAL ARTICLE

Varghese V, Magnani L, Harada N, Mauri FA, Lam EW, Kenny LMet al., 2014, Forkhead box transciption factor M1 (FOXM1) plays a critical role in colorectal cancer resistance by regulating thymidylate synthase (TS), 105th Annual Meeting of the American-Association-for-Cancer-Research (AACR), Publisher: AMER ASSOC CANCER RESEARCH, ISSN: 0008-5472

CONFERENCE PAPER

Magnani L, Stoeck A, Zhang X, Lanczky A, Mirabella AC, Wang T-L, Gyorffy B, Lupien Met al., 2013, Genome-wide reprogramming of the chromatin landscape underlies endocrine therapy resistance in breast cancer, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 110, Pages: E1490-E1499, ISSN: 0027-8424

JOURNAL ARTICLE

Magnani L, Brunelle M, Gevry N, Lupien Met al., 2012, Chromatin landscape and endocrine response in breast cancer, EPIGENOMICS, Vol: 4, Pages: 675-683, ISSN: 1750-1911

JOURNAL ARTICLE

Magnani L, Carroll J, Zwart W, Palmieri Cet al., 2012, ChIPing away at breast cancer, LANCET ONCOLOGY, Vol: 13, Pages: 1185-1187, ISSN: 1470-2045

JOURNAL ARTICLE

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