Imperial College London
Alternate

ProfessorLucaMagnani

Faculty of Medicine, Department of Surgery & Cancer

Honorary Principal Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 2808l.magnani CV

 
 
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Location

 

137ICTEM buildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Cresswell:2020:10.1038/s41467-020-15047-9,
author = {Cresswell, GD and Nichol, D and Spiteri, I and Tari, H and Zapata, L and Heide, T and Maley, CC and Magnani, L and Schiavon, G and Ashworth, A and Barry, P and Sottoriva, A},
doi = {10.1038/s41467-020-15047-9},
journal = {Nature Communications},
pages = {1--12},
title = {Mapping the breast cancer metastatic cascade onto ctDNA using genetic and epigenetic clonal tracking},
url = {http://dx.doi.org/10.1038/s41467-020-15047-9},
volume = {11},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Circulating tumour DNA (ctDNA) allows tracking of the evolution of human cancers at high resolution, overcoming many limitations of tissue biopsies. However, exploiting ctDNA to determine how a patient’s cancer is evolving in order to aid clinical decisions remains difficult. This is because ctDNA is a mix of fragmented alleles, and the contribution of different cancer deposits to ctDNA is largely unknown. Profiling ctDNA almost invariably requires prior knowledge of what genomic alterations to track. Here, we leverage on a rapid autopsy programme to demonstrate that unbiased genomic characterisation of several metastatic sites and concomitant ctDNA profiling at whole-genome resolution reveals the extent to which ctDNA is representative of widespread disease. We also present a methylation profiling method that allows tracking evolutionary changes in ctDNA at single-molecule resolution without prior knowledge. These results have critical implications for the use of liquid biopsies to monitor cancer evolution in humans and guide treatment.
AU  - Cresswell,GD
AU  - Nichol,D
AU  - Spiteri,I
AU  - Tari,H
AU  - Zapata,L
AU  - Heide,T
AU  - Maley,CC
AU  - Magnani,L
AU  - Schiavon,G
AU  - Ashworth,A
AU  - Barry,P
AU  - Sottoriva,A
DO  - 10.1038/s41467-020-15047-9
EP  - 12
PY  - 2020///
SN  - 2041-1723
SP  - 1
TI  - Mapping the breast cancer metastatic cascade onto ctDNA using genetic and epigenetic clonal tracking
T2  - Nature Communications
UR  - http://dx.doi.org/10.1038/s41467-020-15047-9
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000522437200001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://www.nature.com/articles/s41467-020-15047-9
UR  - http://hdl.handle.net/10044/1/84628
VL  - 11
ER  -
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