Imperial College London
Alternate

ProfessorLucaMagnani

Faculty of Medicine, Department of Surgery & Cancer

Honorary Principal Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 2808l.magnani CV

 
 
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Location

 

137ICTEM buildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Zhang:2016:10.1021/acsnano.5b05211,
author = {Zhang, Y and Clausmeyer, J and Babakinejad, B and López, Córdoba A and Ali, T and Shevchuk, A and Takahashi, Y and Novak, P and Edwards, C and Lab, M and Gopal, S and Chiappini, C and Anand, U and Magnani, L and Coombes, RC and Gorelik, J and Matsue, T and Schuhmann, W and Klenerman, D and Sviderskaya, EV and Korchev, Y},
doi = {10.1021/acsnano.5b05211},
journal = {ACS Nano},
pages = {3214--3221},
title = {Spearhead Nanometric Field-Effect Transistor Sensors for Single-Cell Analysis.},
url = {http://dx.doi.org/10.1021/acsnano.5b05211},
volume = {10},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Nanometric field-effect-transistor (FET) sensors are made on the tip of spear-shaped dual carbon nanoelectrodes derived from carbon deposition inside double-barrel nanopipettes. The easy fabrication route allows deposition of semiconductors or conducting polymers to comprise the transistor channel. A channel from electrodeposited poly pyrrole (PPy) exhibits high sensitivity toward pH changes. This property is exploited by immobilizing hexokinase on PPy nano-FETs to give rise to a selective ATP biosensor. Extracellular pH and ATP gradients are key biochemical constituents in the microenvironment of living cells; we monitor their real-time changes in relation to cancer cells and cardiomyocytes. The highly localized detection is possible because of the high aspect ratio and the spear-like design of the nano-FET probes. The accurately positioned nano-FET sensors can detect concentration gradients in three-dimensional space, identify biochemical properties of a single living cell, and after cell membrane penetration perform intracellular measurements.
AU  - Zhang,Y
AU  - Clausmeyer,J
AU  - Babakinejad,B
AU  - López,Córdoba A
AU  - Ali,T
AU  - Shevchuk,A
AU  - Takahashi,Y
AU  - Novak,P
AU  - Edwards,C
AU  - Lab,M
AU  - Gopal,S
AU  - Chiappini,C
AU  - Anand,U
AU  - Magnani,L
AU  - Coombes,RC
AU  - Gorelik,J
AU  - Matsue,T
AU  - Schuhmann,W
AU  - Klenerman,D
AU  - Sviderskaya,EV
AU  - Korchev,Y
DO  - 10.1021/acsnano.5b05211
EP  - 3221
PY  - 2016///
SN  - 1936-086X
SP  - 3214
TI  - Spearhead Nanometric Field-Effect Transistor Sensors for Single-Cell Analysis.
T2  - ACS Nano
UR  - http://dx.doi.org/10.1021/acsnano.5b05211
UR  - http://hdl.handle.net/10044/1/29918
VL  - 10
ER  -
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