Imperial College London
Alternate

ProfessorLuisAragon Alcaide

Faculty of Medicine, Institute of Clinical Sciences

Professor of Genetics
 
 
 
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Contact

 

+44 (0)20 3313 8013luis.aragon

 
 
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Location

 

CRB (Clinical Research Building)Hammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Ramos:2017:10.1007/978-1-4939-6502-1_17,
author = {Ramos, F and Leonard, J and Clemente-Blanco, A and Aragón, L},
doi = {10.1007/978-1-4939-6502-1_17},
journal = {Methods Mol Biol},
pages = {229--243},
title = {Cdc14 and Chromosome Condensation: Evaluation of the Recruitment of Condensin to Genomic Regions.},
url = {http://dx.doi.org/10.1007/978-1-4939-6502-1_17},
volume = {1505},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Chromosome condensation is an essential morphological event required for successful DNA segregation during mitosis. The high level of genome compaction achieved during this process is attained by the evolutionary conserved condensin complex. Recently, several lines of evidences have demonstrated that the mitotic phosphatase Cdc14 is required to ensure condensin loading onto chromosomes. To date several approaches have been used in order to characterize condensin activity and regulation, however these techniques are time-consuming and require complex equipment. In this chapter we described an easy and reliable protocol to analyze Cdc14-dependent condensin loading onto specific genomic DNA regions by using a chromatin immunoprecipitation (ChIP) technique.
AU  - Ramos,F
AU  - Leonard,J
AU  - Clemente-Blanco,A
AU  - Aragón,L
DO  - 10.1007/978-1-4939-6502-1_17
EP  - 243
PY  - 2017///
SP  - 229
TI  - Cdc14 and Chromosome Condensation: Evaluation of the Recruitment of Condensin to Genomic Regions.
T2  - Methods Mol Biol
UR  - http://dx.doi.org/10.1007/978-1-4939-6502-1_17
UR  - https://www.ncbi.nlm.nih.gov/pubmed/27826868
VL  - 1505
ER  -
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