Imperial College London
Alternate

ProfessorMauricioBarahona

Faculty of Natural Sciences, Department of Mathematics

Director of Research, Chair in Biomathematics
 
 
 
//

Contact

 

m.barahona Website

 
 
//

Location

 

6M31Huxley BuildingSouth Kensington Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Noseda:2015:10.1038/ncomms7930,
author = {Noseda, M and Harada, M and McSweeney, S and Leja, T and Belian, E and Stuckey, DJ and Abreu, Paiva MS and Habib, J and Macaulay, I and de, Smith AJ and Al-Beidh, F and Sampson, R and Lumbers, RT and Rao, P and Harding, SE and Blakemore, AI and Eirik, Jacobsen S and Barahona, M and Schneider, MD},
doi = {10.1038/ncomms7930},
journal = {Nature Communications},
title = {PDGFRα demarcates the cardiogenic clonogenic Sca1(+) stem/progenitor cell in adult murine myocardium},
url = {http://dx.doi.org/10.1038/ncomms7930},
volume = {6},
year = {2015}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Cardiac progenitor/stem cells in adult hearts represent an attractive therapeutic target for heart regeneration, though (inter)-relationships among reported cells remain obscure. Using single-cell qRT-PCR and clonal analyses, here we define four subpopulations of cardiac progenitor/stem cells in adult mouse myocardium all sharing stem cell antigen-1 (Sca1), based on side population (SP) phenotype, PECAM-1 (CD31) and platelet-derived growth factor receptor-α (PDGFRα) expression. SP status predicts clonogenicity and cardiogenic gene expression (Gata4/6, Hand2 and Tbx5/20), properties segregating more specifically to PDGFRα(+) cells. Clonal progeny of single Sca1(+) SP cells show cardiomyocyte, endothelial and smooth muscle lineage potential after cardiac grafting, augmenting cardiac function although durable engraftment is rare. PDGFRα(-) cells are characterized by Kdr/Flk1, Cdh5, CD31 and lack of clonogenicity. PDGFRα(+)/CD31(-) cells derive from cells formerly expressing Mesp1, Nkx2-5, Isl1, Gata5 and Wt1, distinct from PDGFRα(-)/CD31(+) cells (Gata5 low; Flk1 and Tie2 high). Thus, PDGFRα demarcates the clonogenic cardiogenic Sca1(+) stem/progenitor cell.
AU  - Noseda,M
AU  - Harada,M
AU  - McSweeney,S
AU  - Leja,T
AU  - Belian,E
AU  - Stuckey,DJ
AU  - Abreu,Paiva MS
AU  - Habib,J
AU  - Macaulay,I
AU  - de,Smith AJ
AU  - Al-Beidh,F
AU  - Sampson,R
AU  - Lumbers,RT
AU  - Rao,P
AU  - Harding,SE
AU  - Blakemore,AI
AU  - Eirik,Jacobsen S
AU  - Barahona,M
AU  - Schneider,MD
DO  - 10.1038/ncomms7930
PY  - 2015///
SN  - 2041-1723
TI  - PDGFRα demarcates the cardiogenic clonogenic Sca1(+) stem/progenitor cell in adult murine myocardium
T2  - Nature Communications
UR  - http://dx.doi.org/10.1038/ncomms7930
UR  - http://hdl.handle.net/10044/1/23263
VL  - 6
ER  -
Download