Imperial College London
Alternate

ProfessorMaria-GloriaBasanez

Faculty of Medicine, School of Public Health

Professor of Neglected Tropical Diseases
 
 
 
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Contact

 

+44 (0)20 7594 3295m.basanez Website

 
 
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Location

 

503School of Public HealthWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Crainey:2017:10.3389/fmicb.2017.00852,
author = {Crainey, JL and Hurst, J and Lamberton, PHL and Cheke, RA and Griffin, CE and Wilson, MD and Mendes, de Araújo CP and Basanez, MG and Post, RJ},
doi = {10.3389/fmicb.2017.00852},
journal = {Frontiers in Microbiology},
title = {The genomic architecture of novel simulium damnosum Wolbachia prophage sequence elements and implications for onchocerciasis epidemiology},
url = {http://dx.doi.org/10.3389/fmicb.2017.00852},
volume = {8},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Research interest in Wolbachia is growing as new discoveries and technical advancements reveal the public health importance of both naturally occurring and artificial infections. Improved understanding of the Wolbachia bacteriophages (WOs) WOcauB2 and WOcauB3 (belonging to a sub-group of four WOs encoding serine recombinases group 1 (sr1WOs)), has enhanced the prospect of novel tools for the genetic manipulation of Wolbachia. The basic biology of sr1WOs, including host range and mode of genomic integration is, however, still poorly understood. Very few sr1WOs have been described, with two such elements putatively resulting from integrations at the same Wolbachia genome loci, about 2 kb downstream from the FtsZ cell-division gene. Here we characterise the DNA sequence flanking the FtsZ gene of wDam, a genetically distinct line of Wolbachia isolated from the West African onchocerciasis vector Simulium squamosum E. Using Roche 454 shot-gun and Sanger sequencing, we have resolved >32 kb of WO prophage sequence into 3 contigs representing three distinct prophage elements. Spanning ≥ 36 distinct WO open reading frame gene sequences, these prophage elements correspond roughly to three different WO modules: a serine recombinase and replication module (sr1RRM), a head and base-plate module and a tail module. The sr1RRM module contains replication genes and a Holliday junction recombinase and is unique to the sr1 group WOs. In the extreme terminal of the tail module there is an SpvB protein homologue—believed to have insecticidal properties and proposed to have a role in how Wolbachia parasitize their insect hosts. We propose that these wDam prophage modules all derive from a single WO genome, which we have named here sr1WOdamA1. The best-match database sequence for all of our sr1WOdamA1-predicted gene sequences was annotated as of Wolbachia or Wolbachia phage sourced from an arthropod. Clear evidence of exchange between sr1WOdamA1 and other Wolbachia WO phage seq
AU  - Crainey,JL
AU  - Hurst,J
AU  - Lamberton,PHL
AU  - Cheke,RA
AU  - Griffin,CE
AU  - Wilson,MD
AU  - Mendes,de Araújo CP
AU  - Basanez,MG
AU  - Post,RJ
DO  - 10.3389/fmicb.2017.00852
PY  - 2017///
SN  - 1664-302X
TI  - The genomic architecture of novel simulium damnosum Wolbachia prophage sequence elements and implications for onchocerciasis epidemiology
T2  - Frontiers in Microbiology
UR  - http://dx.doi.org/10.3389/fmicb.2017.00852
UR  - http://www.imperial.ac.uk/people/m.basanez
UR  - http://www.frontiersin.org/
UR  - http://hdl.handle.net/10044/1/48327
VL  - 8
ER  -
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