Imperial College London

DrMorganBeeby

Faculty of Natural SciencesDepartment of Life Sciences

Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 5251m.beeby Website

 
 
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Location

 

502Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
to

37 results found

Chaban B, Coleman I, Beeby M, 2018, Evolution of higher torque in Campylobacter-type bacterial flagellar motors, SCIENTIFIC REPORTS, Vol: 8, ISSN: 2045-2322

JOURNAL ARTICLE

Henderson LD, Beeby M, 2018, High-Throughput Electron Cryo-tomography of Protein Complexes and Their Assembly., Methods Mol Biol, Vol: 1764, Pages: 29-44

Electron cryo-tomography and subtomogram averaging enable visualization of protein complexes in situ, in three dimensions, in a near-native frozen-hydrated state to nanometer resolutions. To achieve this, intact cells are vitrified and imaged over a range of tilts within an electron microscope. These images can subsequently be reconstructed into a three-dimensional volume representation of the sample cell. Because complexes are visualized in situ, crucial insights into their mechanism, assembly process, and dynamic interactions with other proteins become possible. To illustrate the electron cryo-tomography workflow for visualizing protein complexes in situ, we describe our workflow of preparing samples, imaging, and image processing using Leginon for data collection, IMOD for image reconstruction, and PEET for subtomogram averaging.

JOURNAL ARTICLE

Rossmann FM, Beeby M, 2018, Insights into the evolution of bacterial flagellar motors from high-throughput in situ electron cryotomography and subtomogram averaging, ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, Vol: 74, Pages: 585-594, ISSN: 2059-7983

JOURNAL ARTICLE

Thomson NM, Rossmann FM, Ferreira JL, Matthews-Palmer TR, Beeby M, Pallen MJet al., 2018, Bacterial Flagellins: Does Size Matter?, TRENDS IN MICROBIOLOGY, Vol: 26, Pages: 575-581, ISSN: 0966-842X

JOURNAL ARTICLE

Asmar AT, Ferreira JL, Cohen EJ, Cho S-H, Beeby M, Hughes KT, Collet J-Fet al., 2017, Communication across the bacterial cell envelope depends on the size of the periplasm, PLOS BIOLOGY, Vol: 15, ISSN: 1545-7885

JOURNAL ARTICLE

Cohen EJ, Ferreira JL, Ladinsky MS, Beeby M, Hughes KTet al., 2017, Nanoscale-length control of the flagellar driveshaft requires hitting the tethered outer membrane, SCIENCE, Vol: 356, Pages: 197-200, ISSN: 0036-8075

JOURNAL ARTICLE

Ferreira J, Matthews-Palmer T, Beeby M, 2017, Electron cryo-tomography, Cellular Imaging Electron Tomography and Related Techniques, Editors: Hanssen, Publisher: Springer, ISBN: 9783319689975

This book highlights important techniques for cellular imaging and covers the basics and applications of electron tomography and related techniques.

BOOK CHAPTER

Haurat MF, Figueiredo AS, Hoffmann L, Li L, Herr K, Wilson AJ, Beeby M, Schaber J, Albers S-Vet al., 2017, ArnS, a kinase involved in starvation-induced archaellum expression, MOLECULAR MICROBIOLOGY, Vol: 103, Pages: 181-194, ISSN: 0950-382X

JOURNAL ARTICLE

Hoffmann L, Schummer A, Reimann J, Haurat MF, Wilson AJ, Beeby M, Warscheid B, Albers S-Vet al., 2017, Expanding the archaellum regulatory network - the eukaryotic protein kinases ArnC and ArnD influence motility of Sulfolobus acidocaldarius, MICROBIOLOGYOPEN, Vol: 6, ISSN: 2045-8827

JOURNAL ARTICLE

Thomson NM, Ferreira J, Matthews-Palmer T, Beeby M, Pallen Met al., 2017, Giant flagellins form thick flagellar filaments in two species of marine Gammaproteobacteria

Flagella, the primary means of motility in bacteria, contain thousands of copies of the protein flagellin in self-assembled helical filaments that function as microscopic propellers. Evolution has presented a wide range of different sizes of flagellin, but the upper reaches of the size distribution have barely been explored. We show here that two species of marine Gammaproteobacteria, Bermanella marisrubri Red65 and Oleivorans marinus 2O1, are motile due to the production of thick, monopolar flagellar filaments. In each case, a 'giant' flagellin of more than 1,000 amino acids is the only predicted flagellin protein. Two species of Methylobacterium from the leaves of Arabidopsis thaliana also both possess genes for giant flagellins. However, their flagellar filaments are of similar thickness to bacteria with flagellins half the size. This may be explained by the presence of multiple, smaller, flagellin genes in the Methylobacterium species. This work further illustrates how "the" bacterial flagellum is not a single, ideal structure, but a continuum of evolved machines adapted to a wide range of niches.

JOURNAL ARTICLE

Yao Q, Jewett AI, Chang Y-W, Oikonomou CM, Beeby M, Iancu CV, Briegel A, Ghosal D, Jensen GJet al., 2017, Short FtsZ filaments can drive asymmetric cell envelope constriction at the onset of bacterial cytokinesis, EMBO JOURNAL, Vol: 36, Pages: 1577-1589, ISSN: 0261-4189

JOURNAL ARTICLE

Beeby M, Ribardo DA, Brennan CA, Ruby EG, Jensen GJ, Hendrixson DRet al., 2016, Diverse high-torque bacterial flagellar motors assemble wider stator rings using a conserved protein scaffold, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 113, Pages: E1917-E1926, ISSN: 0027-8424

JOURNAL ARTICLE

Nguyen LT, Swulius M, Gumbart JC, Beeby M, Jensen GJet al., 2016, Coarse-Grained Simulations Reveal Mechanisms of Bacterial Morphogenesis, 60th Annual Meeting of the Biophysical-Society, Publisher: CELL PRESS, Pages: 468A-468A, ISSN: 0006-3495

CONFERENCE PAPER

Oikonomou CM, Swulius MT, Briegel A, Beeby M, Yao Q, Chang Y-W, Jensen GJet al., 2016, Electron cryotomography, Editors: Harwood, Jensen, Publisher: ELSEVIER ACADEMIC PRESS INC, Pages: 115-139

BOOK CHAPTER

Taylor WR, Matthews-Palmer TRS, Beeby M, 2016, Molecular Models for the Core Components of the Flagellar Type-III Secretion Complex, PLOS ONE, Vol: 11, ISSN: 1932-6203

JOURNAL ARTICLE

Beeby M, 2015, Motility in the epsilon-proteobacteria, CURRENT OPINION IN MICROBIOLOGY, Vol: 28, Pages: 115-121, ISSN: 1369-5274

JOURNAL ARTICLE

Chaban B, Hughes HV, Beeby M, 2015, The flagellum in bacterial pathogens: For motility and a whole lot more, SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, Vol: 46, Pages: 91-103, ISSN: 1084-9521

JOURNAL ARTICLE

Nguyen LT, Gumbart JC, Beeby M, Jensen GJet al., 2015, Coarse-grained simulations of bacterial cell wall growth reveal that local coordination alone can be sufficient to maintain rod shape, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 112, Pages: E3689-E3698, ISSN: 0027-8424

JOURNAL ARTICLE

Beeby M, 2014, Evolution of Novel Components of the Bacterial Flagellar Motor, 28th Annual Symposium of the Protein-Society, Publisher: WILEY-BLACKWELL, Pages: 61-61, ISSN: 0961-8368

CONFERENCE PAPER

Gumbart JC, Beeby M, Jensen GJ, Roux Bet al., 2014, Escherichia coli Peptidoglycan Structure and Mechanics as Predicted by Atomic-Scale Simulations, PLOS COMPUTATIONAL BIOLOGY, Vol: 10, ISSN: 1553-734X

JOURNAL ARTICLE

Mueller A, Beeby M, McDowall AW, Chow J, Jensen GJ, Clemons WMet al., 2014, Ultrastructure and complex polar architecture of the human pathogen Campylobacter jejuni, MICROBIOLOGYOPEN, Vol: 3, Pages: 702-710, ISSN: 2045-8827

JOURNAL ARTICLE

Abrusci P, Vergara-Irigaray M, Johnson S, Beeby MD, Hendrixson DR, Roversi P, Friede ME, Deane JE, Jensen GJ, Tang CM, Lea SMet al., 2013, Architecture of the major component of the type III secretion system export apparatus, NATURE STRUCTURAL & MOLECULAR BIOLOGY, Vol: 20, Pages: 99-U126, ISSN: 1545-9993

JOURNAL ARTICLE

Beeby M, Gumbart JC, Roux B, Jensen GJet al., 2013, Architecture and assembly of the Gram-positive cell wall, MOLECULAR MICROBIOLOGY, Vol: 88, Pages: 664-672, ISSN: 0950-382X

JOURNAL ARTICLE

Jensen G, Briegel A, Beeby M, 2013, Visualizing large macromolecular assemblies in vivo with electron cryotomography, 245th National Spring Meeting of the American-Chemical-Society (ACS), Publisher: AMER CHEMICAL SOC, ISSN: 0065-7727

CONFERENCE PAPER

Beeby M, Cho M, Stubbe J, Jensen GJet al., 2012, Growth and Localization of Polyhydroxybutyrate Granules in Ralstonia eutropha, JOURNAL OF BACTERIOLOGY, Vol: 194, Pages: 1092-1099, ISSN: 0021-9193

JOURNAL ARTICLE

Briegel A, Beeby M, Thanbichler M, Jensen GJet al., 2011, Activated chemoreceptor arrays remain intact and hexagonally packed, MOLECULAR MICROBIOLOGY, Vol: 82, Pages: 748-757, ISSN: 0950-382X

JOURNAL ARTICLE

Chen S, Beeby M, Murphy GE, Leadbetter JR, Hendrixson DR, Briegel A, Li Z, Shi J, Tocheva EI, Mueller A, Dobro MJ, Jensen GJet al., 2011, Structural diversity of bacterial flagellar motors, EMBO JOURNAL, Vol: 30, Pages: 2972-2981, ISSN: 0261-4189

JOURNAL ARTICLE

Chen S, McDowall A, Dobro MJ, Briegel A, Ladinsky M, Shi J, Tocheva EI, Beeby M, Pilhofer M, Ding HJ, Li Z, Gan L, Morris DM, Jensen GJet al., 2010, Electron cryotomography of bacterial cells., J Vis Exp

While much is already known about the basic metabolism of bacterial cells, many fundamental questions are still surprisingly unanswered, including for instance how they generate and maintain specific cell shapes, establish polarity, segregate their genomes, and divide. In order to understand these phenomena, imaging technologies are needed that bridge the resolution gap between fluorescence light microscopy and higher-resolution methods such as X-ray crystallography and NMR spectroscopy. Electron cryotomography (ECT) is an emerging technology that does just this, allowing the ultrastructure of cells to be visualized in a near-native state, in three dimensions (3D), with "macromolecular" resolution (approximately 4nm).(1, 2) In ECT, cells are imaged in a vitreous, "frozen-hydrated" state in a cryo transmission electron microscope (cryoTEM) at low temperature (< -180 degrees C). For slender cells (up to approximately 500 nm in thickness(3)), intact cells are plunge-frozen within media across EM grids in cryogens such as ethane or ethane/propane mixtures. Thicker cells and biofilms can also be imaged in a vitreous state by first "high-pressure freezing" and then, "cryo-sectioning" them. A series of two-dimensional projection images are then collected through the sample as it is incrementally tilted along one or two axes. A three-dimensional reconstruction, or "tomogram" can then be calculated from the images. While ECT requires expensive instrumentation, in recent years, it has been used in a few labs to reveal the structures of various external appendages, the structures of different cell envelopes, the positions and structures of cytoskeletal filaments, and the locations and architectures of large macromolecular assemblies such as flagellar motors, internal compartments and chemoreceptor arrays.(1, 2) In this video article we illustrate how to image cells with ECT, including the processes of sample preparation, dat

JOURNAL ARTICLE

Beeby M, Bobik TA, Yeates TO, 2009, Exploiting genomic patterns to discover new supramolecular protein assemblies, PROTEIN SCIENCE, Vol: 18, Pages: 69-79, ISSN: 0961-8368

JOURNAL ARTICLE

Chim N, McMath LM, Beeby M, Goulding CWet al., 2009, Advances in Mycobacterium tuberculosis structural genomics: investigating potential chinks in the armor of a deadly pathogen, Infect Disord Drug Targets, Vol: 9, Pages: 475-492

JOURNAL ARTICLE

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