Imperial College London
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DrMorganBeeby

Faculty of Natural Sciences, Department of Life Sciences

Reader in Structural Biology
 
 
 
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Contact

 

m.beeby Website

 
 
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Location

 

502Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Cohen:2019:10.1101/2019.12.26.888701,
author = {Cohen, EJ and Nakane, D and Kabata, Y and Hendrixson, DR and Nishizaka, T and Beeby, M},
doi = {10.1101/2019.12.26.888701},
title = {“<i>Campylobacter jejuni</i>motility integrates specialized cell shape, flagellar filament, and motor, to coordinate action of its opposed flagella in viscous media”},
url = {http://dx.doi.org/10.1101/2019.12.26.888701},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - <jats:title>Abstract</jats:title><jats:p><jats:italic>Campylobacter jejuni</jats:italic>rotates a flagellum at each pole to swim through the viscous mucosa of its hosts’ gastrointestinal tracts. Despite their importance for host colonization, however, how<jats:italic>C. jejuni</jats:italic>coordinates rotation of these two opposing flagella is unclear. As well as their polar placement,<jats:italic>C. jejuni’s</jats:italic>flagella deviate from the Enterobacteriaceael norm in other ways: their flagellar motors produce much higher torque and their flagellar filament is made of two different zones of two different flagellins. To understand how<jats:italic>C. jejuni’s</jats:italic>opposed motors coordinate, and what contribution these factors play in<jats:italic>C. jejuni</jats:italic>motility, we developed strains with flagella that could be fluorescently labeled, and observed them by high-speed video microscopy. We found that<jats:italic>C. jejuni</jats:italic>coordinates its dual flagella by wrapping the leading filament around the cell body during swimming in high-viscosity media and that its differentiated flagellar filament has evolved to facilitate this wrapped-mode swimming. Unexpectedly,<jats:italic>C. jejuni</jats:italic>’s helical body is important for facile unwrapping of the flagellar filament from the cell body during switching of swimming trajectory. Our findings demonstrate how multiple facets of<jats:italic>C. jejuni’s</jats:italic>flagella and cell plan have co-evolved for optimal motility in high-viscosity environments.</jats:p>
AU  - Cohen,EJ
AU  - Nakane,D
AU  - Kabata,Y
AU  - Hendrixson,DR
AU  - Nishizaka,T
AU  - Beeby,M
DO  - 10.1101/2019.12.26.888701
PY  - 2019///
TI  - “<i>Campylobacter jejuni</i>motility integrates specialized cell shape, flagellar filament, and motor, to coordinate action of its opposed flagella in viscous media”
UR  - http://dx.doi.org/10.1101/2019.12.26.888701
ER  -
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