Imperial College London
Alternate

ProfessorMarinaBotto

Faculty of Medicine, Department of Immunology and Inflammation

Head of Department, Director of Bioservices
 
 
 
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Contact

 

+44 (0)20 3313 2316m.botto Website

 
 
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Assistant

 

Miss Claudia Rocchi +44 (0)20 3313 2315

 
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Location

 

9N10Commonwealth BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Kraivong:2013:10.1111/cei.12184,
author = {Kraivong, R and Vasanawathana, S and Limpitikul, W and Malasit, P and Tangthawornchaikul, N and Botto, M and Screaton, GR and Mongkolsapaya, J and Pickering, MC},
doi = {10.1111/cei.12184},
journal = {Clinical and Experimental Immunology},
pages = {326--334},
title = {Complement alternative pathway genetic variation and Dengue infection in the Thai population},
url = {http://dx.doi.org/10.1111/cei.12184},
volume = {174},
year = {2013}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Dengue disease is a mosquito-borne infection caused by Dengue virus. Infection may be asymptomatic or variably manifest as mild Dengue fever (DF) to the most severe form, Dengue haemorrhagic fever (DHF). Mechanisms that influence disease severity are not understood. Complement, an integral component of the immune system, is activated during Dengue infection and the degree of activation increases with disease severity. Activation of the complement alternative pathway is influenced by polymorphisms within activation (factor B rs12614/rs641153, C3 rs2230199) and regulatory [complement factor H (CFH) rs800292] proteins, collectively termed a complotype. Here, we tested the hypothesis that the complotype influences disease severity during secondary Dengue infection. In addition to the complotype, we also assessed two other disease-associated CFH polymorphisms (rs1061170, rs3753394) and a structural polymorphism within the CFH protein family. We did not detect any significant association between the examined polymorphisms and Dengue infection severity in the Thai population. However, the minor allele frequencies of the factor B and C3 polymorphisms were less than 10%, so our study was not sufficiently powered to detect an association at these loci. We were also unable to detect a direct interaction between CFH and Dengue NS1 using both recombinant NS1 and DV2-infected culture supernatants. We conclude that the complotype does not influence secondary Dengue infection severity in the Thai population.
AU  - Kraivong,R
AU  - Vasanawathana,S
AU  - Limpitikul,W
AU  - Malasit,P
AU  - Tangthawornchaikul,N
AU  - Botto,M
AU  - Screaton,GR
AU  - Mongkolsapaya,J
AU  - Pickering,MC
DO  - 10.1111/cei.12184
EP  - 334
PY  - 2013///
SN  - 1365-2249
SP  - 326
TI  - Complement alternative pathway genetic variation and Dengue infection in the Thai population
T2  - Clinical and Experimental Immunology
UR  - http://dx.doi.org/10.1111/cei.12184
UR  - http://hdl.handle.net/10044/1/24063
VL  - 174
ER  -
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