Imperial College London
Alternate

ProfessorMartynBoutelle

Faculty of Engineering, Department of Bioengineering

Associate Provost (Estates Planning)
 
 
 
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Contact

 

+44 (0)20 7594 5138m.boutelle Website CV

 
 
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Location

 

B208Bessemer BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Booth:2021:10.1021/acs.analchem.0c05108,
author = {Booth, MA and Gowers, SAN and Hersey, M and Samper, IC and Park, S and Anikeeva, P and Hashemi, P and Stevens, MM and Boutelle, MG},
doi = {10.1021/acs.analchem.0c05108},
journal = {Analytical Chemistry},
pages = {6646--6655},
title = {Fiber-based electrochemical biosensors for monitoring pH and transient neurometabolic lactate.},
url = {http://dx.doi.org/10.1021/acs.analchem.0c05108},
volume = {93},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Developing tools that are able to monitor transient neurochemical dynamics is important to decipher brain chemistry and function. Multifunctional polymer-based fibers have been recently applied to monitor and modulate neural activity. Here, we explore the potential of polymer fibers comprising six graphite-doped electrodes and two microfluidic channels within a flexible polycarbonate body as a platform for sensing pH and neurometabolic lactate. Electrodes were made into potentiometric sensors (responsive to pH) or amperometric sensors (lactate biosensors). The growth of an iridium oxide layer made the fiber electrodes responsive to pH in a physiologically relevant range. Lactate biosensors were fabricated via platinum black growth on the fiber electrode, followed by an enzyme layer, making them responsive to lactate concentration. Lactate fiber biosensors detected transient neurometabolic lactate changes in an in vivo mouse model. Lactate concentration changes were associated with spreading depolarizations, known to be detrimental to the injured brain. Induced waves were identified by a signature lactate concentration change profile and measured as having a speed of ∼2.7 mm/min (n = 4 waves). Our work highlights the potential applications of fiber-based biosensors for direct monitoring of brain metabolites in the context of injury.
AU  - Booth,MA
AU  - Gowers,SAN
AU  - Hersey,M
AU  - Samper,IC
AU  - Park,S
AU  - Anikeeva,P
AU  - Hashemi,P
AU  - Stevens,MM
AU  - Boutelle,MG
DO  - 10.1021/acs.analchem.0c05108
EP  - 6655
PY  - 2021///
SN  - 0003-2700
SP  - 6646
TI  - Fiber-based electrochemical biosensors for monitoring pH and transient neurometabolic lactate.
T2  - Analytical Chemistry
UR  - http://dx.doi.org/10.1021/acs.analchem.0c05108
UR  - https://www.ncbi.nlm.nih.gov/pubmed/33797893
UR  - https://pubs.acs.org/doi/10.1021/acs.analchem.0c05108
UR  - http://hdl.handle.net/10044/1/88197
VL  - 93
ER  -
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