Imperial College London
Alternate

Professor Martin Buck FRS

Faculty of Natural Sciences, Department of Life Sciences

Senior Research Investigator
 
 
 
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Contact

 

+44 (0)20 7594 5442m.buck

 
 
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Location

 

448Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Bonato:2016:10.1111/febs.13897,
author = {Bonato, P and Alves, LR and Osaki, JH and Rigo, LU and Pedrosa, FO and Souza, EM and Zhang, N and Schumacher, J and Buck, M and Wassem, R and Chubatsu, LS},
doi = {10.1111/febs.13897},
journal = {FEBS Journal},
pages = {3919--3930},
title = {The NtrY/NtrX two-component system is involved in controlling nitrate assimilation in Herbaspirillum seropedicae strain SmR1.},
url = {http://dx.doi.org/10.1111/febs.13897},
volume = {283},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Herbaspirillum seropedicae is a diazotrophic β-Proteobacterium found endophytically associated with gramineae (Poaceae or graminaceous plants) such as rice, sorghum and sugar cane. In this work we show that nitrate-dependent growth in this organism is regulated by the master nitrogen regulatory two-component system NtrB/NtrC, and by NtrY/NtrX which functions to specifically regulate nitrate metabolism. NtrY is a histidine kinase sensor protein predicted to be associated with the membrane and NtrX is the response regulator partner. The ntrYntrX genes are widely distributed in Proteobacteria. In α-Proteobacteria they are frequently located downstream from ntrBC, whereas in β-Proteobacteria these genes are located downstream from genes encoding a RNA methyltransferase and a proline-rich protein with unknown function. The α-Proteobacteria NtrX protein has an AAA+ domain, absent in those from β-Proteobacteria. An ntrY mutant of H. seropedicae showed wild type fixing nitrogen phenotype, but the nitrate dependent growth was abolished. Gene fusion assays indicated that NtrY is involved in the expression of genes coding for the assimilatory nitrate reductase as well as the nitrate-responsive two-component system NarX/NarL (narK and narX promoters, respectively). The purified NtrX protein was capable of binding the narK and narX promoters, and the binding site at the narX promoter for the NtrX protein was determined by DNA footprinting. In silico analyses revealed similar sequences in other promoter regions of H. seropedicae that are related to nitrate assimilation, supporting the role of the NtrY/NtrX system in regulating nitrate metabolism in H. seropedicae. This article is protected by copyright. All rights reserved.
AU  - Bonato,P
AU  - Alves,LR
AU  - Osaki,JH
AU  - Rigo,LU
AU  - Pedrosa,FO
AU  - Souza,EM
AU  - Zhang,N
AU  - Schumacher,J
AU  - Buck,M
AU  - Wassem,R
AU  - Chubatsu,LS
DO  - 10.1111/febs.13897
EP  - 3930
PY  - 2016///
SN  - 1742-4658
SP  - 3919
TI  - The NtrY/NtrX two-component system is involved in controlling nitrate assimilation in Herbaspirillum seropedicae strain SmR1.
T2  - FEBS Journal
UR  - http://dx.doi.org/10.1111/febs.13897
UR  - http://hdl.handle.net/10044/1/45475
VL  - 283
ER  -
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