Imperial College London

ProfessorMichaelSchneider

Faculty of MedicineNational Heart & Lung Institute

Chair in Cardiology
 
 
 
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Contact

 

+44 (0)013 34621727m.d.schneider Website

 
 
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Location

 

ICTEM buildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Payne:1987:10.1073/pnas.84.24.8956,
author = {Payne, PA and Olson, EN and Hsiau, P and Roberts, R and Perryman, MB and Schneider, MD},
doi = {10.1073/pnas.84.24.8956},
journal = {Proc Natl Acad Sci U S A},
pages = {8956--8960},
title = {An activated c-Ha-ras allele blocks the induction of muscle-specific genes whose expression is contingent on mitogen withdrawal.},
url = {http://dx.doi.org/10.1073/pnas.84.24.8956},
volume = {84},
year = {1987}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - During myogenesis, induction of muscle-specific genes is subject to negative control by polypeptide mitogens and type-beta transforming growth factor. Since transduction of growth factor signals may require proteins encoded by cellular ras oncogenes, we have tested whether a mutationally altered Harvey ras expression vector, by itself, can prevent establishment of a differentiated phenotype in BC3H1 mouse myoblasts. Transfection with the valine-12 allele of the human Harvey ras gene, under the control of its own promoter, was sufficient to prevent the induction of both muscle creatine kinase activity and the nicotinic acetylcholine receptor following mitogen withdrawal but did not inhibit withdrawal from the cell cycle. The loss of creatine kinase activity resulted from a corresponding block to induction of muscle creatine kinase mRNA. Similarly, mitogen withdrawal elicited little or no alpha-actin mRNA in ras-transfected cells. These results suggest that an activated ras allele can inhibit myogenesis through a mechanism independent of cell proliferation and can preclude activation of genes whose up-regulation normally accompanies mitogen withdrawal.
AU - Payne,PA
AU - Olson,EN
AU - Hsiau,P
AU - Roberts,R
AU - Perryman,MB
AU - Schneider,MD
DO - 10.1073/pnas.84.24.8956
EP - 8960
PY - 1987///
SN - 0027-8424
SP - 8956
TI - An activated c-Ha-ras allele blocks the induction of muscle-specific genes whose expression is contingent on mitogen withdrawal.
T2 - Proc Natl Acad Sci U S A
UR - http://dx.doi.org/10.1073/pnas.84.24.8956
UR - https://www.ncbi.nlm.nih.gov/pubmed/3122209
VL - 84
ER -