Imperial College London

ProfessorMichaelSchneider

Faculty of MedicineNational Heart & Lung Institute

Chair in Cardiology
 
 
 
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Contact

 

+44 (0)013 34621727m.d.schneider Website

 
 
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Location

 

ICTEM buildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Noseda:2015:10.1038/ncomms7930,
author = {Noseda, M and Harada, M and McSweeney, S and Leja, T and Belian, E and Stuckey, DJ and Abreu, Paiva MS and Habib, J and Macaulay, I and de, Smith AJ and Al-Beidh, F and Sampson, R and Lumbers, RT and Rao, P and Harding, SE and Blakemore, AI and Eirik, Jacobsen S and Barahona, M and Schneider, MD},
doi = {10.1038/ncomms7930},
journal = {Nature Communications},
pages = {6930--6930},
title = {PDGFRα demarcates the cardiogenic clonogenic Sca1(+) stem/progenitor cell in adult murine myocardium.},
url = {http://dx.doi.org/10.1038/ncomms7930},
volume = {6},
year = {2015}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Cardiac progenitor/stem cells in adult hearts represent an attractive therapeutic target for heart regeneration, though (inter)-relationships among reported cells remain obscure. Using single-cell qRT-PCR and clonal analyses, here we define four subpopulations of cardiac progenitor/stem cells in adult mouse myocardium all sharing stem cell antigen-1 (Sca1), based on side population (SP) phenotype, PECAM-1 (CD31) and platelet-derived growth factor receptor-α (PDGFRα) expression. SP status predicts clonogenicity and cardiogenic gene expression (Gata4/6, Hand2 and Tbx5/20), properties segregating more specifically to PDGFRα(+) cells. Clonal progeny of single Sca1(+) SP cells show cardiomyocyte, endothelial and smooth muscle lineage potential after cardiac grafting, augmenting cardiac function although durable engraftment is rare. PDGFRα(-) cells are characterized by Kdr/Flk1, Cdh5, CD31 and lack of clonogenicity. PDGFRα(+)/CD31(-) cells derive from cells formerly expressing Mesp1, Nkx2-5, Isl1, Gata5 and Wt1, distinct from PDGFRα(-)/CD31(+) cells (Gata5 low; Flk1 and Tie2 high). Thus, PDGFRα demarcates the clonogenic cardiogenic Sca1(+) stem/progenitor cell.
AU - Noseda,M
AU - Harada,M
AU - McSweeney,S
AU - Leja,T
AU - Belian,E
AU - Stuckey,DJ
AU - Abreu,Paiva MS
AU - Habib,J
AU - Macaulay,I
AU - de,Smith AJ
AU - Al-Beidh,F
AU - Sampson,R
AU - Lumbers,RT
AU - Rao,P
AU - Harding,SE
AU - Blakemore,AI
AU - Eirik,Jacobsen S
AU - Barahona,M
AU - Schneider,MD
DO - 10.1038/ncomms7930
EP - 6930
PY - 2015///
SN - 2041-1723
SP - 6930
TI - PDGFRα demarcates the cardiogenic clonogenic Sca1(+) stem/progenitor cell in adult murine myocardium.
T2 - Nature Communications
UR - http://dx.doi.org/10.1038/ncomms7930
UR - http://hdl.handle.net/10044/1/23263
VL - 6
ER -