Imperial College London
Alternate

Emeritus ProfessorMichaelSchneider

Faculty of Medicine, National Heart & Lung Institute

Emeritus Professor in Cardiology
 
 
 
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Contact

 

+44 (0)013 34621727m.d.schneider Website

 
 
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Location

 

ICTEM buildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Mioulane:2012:10.1007/s12265-012-9396-1,
author = {Mioulane, M and Foldes, G and Ali, NN and Schneider, MD and Harding, SE},
doi = {10.1007/s12265-012-9396-1},
journal = {Journal of Cardiovascular Translational Research},
pages = {593--604},
title = {Development of high content imaging methods for cell death detection in human pluripotent stem cell-derived cardiomyocytes},
url = {http://dx.doi.org/10.1007/s12265-012-9396-1},
volume = {5},
year = {2012}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Human pluripotent stem cell-derived cardiomyocytes (hPSC-CM) are being investigated as a new source of cardiac cells for drug safety assessment. We developed a novel scalable high content microscopy-based method for the detection of cell death in hPSC-CM that can serve for future predictive in vitro cardio-toxicological screens. Using rat neonatal ventricular cardiomyocytes (RVNC) or hPSC-CM, assays for nuclear remodelling, mitochondrial status, apoptosis and necrosis were designed using a combination of fluorescent dyes and antibodies on an automated microscopy platform. This allowed the observation of a chelerythrine-induced concentration-dependent apoptosis to necrosis switch and time-dependent progression of early apoptotic cells towards a necrotic-like phenotype. Susceptibility of hPSC-CM to chelerythrine-stimulated apoptosis varied with time after differentiation, but at most time points, hPSC-CM were more resistant than RVNC. This simple and scalable humanized high-content assay generates accurate cardiotoxicity profiles that can serve as a base for further assessment of cardioprotective strategies and drug safety.
AU  - Mioulane,M
AU  - Foldes,G
AU  - Ali,NN
AU  - Schneider,MD
AU  - Harding,SE
DO  - 10.1007/s12265-012-9396-1
EP  - 604
PY  - 2012///
SN  - 1937-5387
SP  - 593
TI  - Development of high content imaging methods for cell death detection in human pluripotent stem cell-derived cardiomyocytes
T2  - Journal of Cardiovascular Translational Research
UR  - http://dx.doi.org/10.1007/s12265-012-9396-1
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000308955500004&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/60596
VL  - 5
ER  -
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