Imperial College London

ProfessorMargaretDallman

Central FacultyOffice of the Provost

Associate Provost (Academic Partnerships)
 
 
 
//

Contact

 

+44 (0)20 7594 5406m.dallman Website

 
 
//

Assistant

 

Ms Emelia Gobbe +44 (0)20 7594 7460

 
//

Location

 

2.11Faculty BuildingSouth Kensington Campus

//

Summary

 

Publications

Publication Type
Year
to

151 results found

Watson T, Andrews N, Davis S, Bugeon L, Dallman MD, McGinty Jet al., 2017, OPTiM: Optical projection tomography integrated microscope using open-source hardware and software, PLOS ONE, Vol: 12, ISSN: 1932-6203

JOURNAL ARTICLE

Andrews N, Ramel M-C, Kumar S, Alexandrov Y, Kelly DJ, Warren SC, Kerry L, Lockwood N, Frolov A, Frankel P, Bugeon L, McGinty J, Dallman MJ, French PMWet al., 2016, Visualising apoptosis in live zebrafish using fluorescence lifetime imaging with optical projection tomography to map FRET biosensor activity in space and time, JOURNAL OF BIOPHOTONICS, Vol: 9, Pages: 414-424, ISSN: 1864-063X

JOURNAL ARTICLE

Jha A, Progatzky F, Wane M, Thwaites RS, McBrien M, Brimley J, Tunstall T, Shattock RJ, Bugeon L, Openshaw PJM, Dallman MJ, Hansel TTet al., 2016, Human nasal mucosal responses to TLR agonists are mirrored by the zebrafish gill, British Association of Lung Research Summer Congress

Introduction: There are few reliable ways to study respiratory mucosal immune responses to viruses, viral-type toll-like receptor (TLR) agonists and vaccines. To investigate innate immune responses to TLR agonists (TLR3: poly IC/ poly ICLC; TLR7/8: resiquimod), we compared the effects on human nasal mucosa and zebrafish gills in vivo. Methods: Nasal challenge of adult volunteers was performed with saline, poly IC (n=4), poly ICLC (n=4) or resiquimod (n=8; 5 non-atopic, 3 atopic). Nasal mucosal lining fluid (MLF) was obtained by nasosorption at regular intervals up to 24 hours after challenge; nasal obstruction was monitored by peak nasal inspiratory flow (PNIF) and total nasal symptom scores (TNSS). Cytokines and interferons were measured in MLF using electrochemiluminescence on the Meso Scale Discovery (MSD) platform. Adult zebrafish gills were exposed to the same TLR agonists and gene expression was quantified in gill tissue at similar time-points. Results: Nasal challenge with TLR3 agonists failed to elicit any significant responses when compared to saline. In contrast resiquimod (10μg/100μl per nostril) caused a potent induction of cytokines with an early release (1-3 hours) of IFN-α2a, TNF-α and IL-1β and a later release (after 4 hours) of IFN-γ. The 3 volunteers with the highest levels of IFN-α2a were atopic. Six volunteers were asymptomatic and two volunteers had flu-like symptoms. There were no significant changes in clinical correlates of nasal obstruction. After resiquimod administration, but not TLR3 agonists, zebrafish gills showed an immune profile remarkably analogous to human nasal responses. Conclusion: The TLR7/8 agonist resiquimod is a potent mucosal inducer of IFN-α2a, IFN-γ and proinflammatory cytokines, whilst TLR3 agonists failed to stimulate mucosal innate immune responses. Zebrafish gills accurately mimic human nasal mucosal responses following exposure to TLR agonists, offering translational app

CONFERENCE PAPER

Kumar S, Lockwood N, Ramel M-C, Correia T, Ellis M, Alexandrov Y, Andrews N, Patel R, Bugeon L, Dallman MJ, Brandner S, Arridge S, Katan M, McGinty J, Frankel P, French PMWet al., 2016, Quantitative in vivo optical tomography of cancer progression & vasculature development in adult zebrafish, ONCOTARGET, Vol: 7, Pages: 43939-43948, ISSN: 1949-2553

JOURNAL ARTICLE

Progatzky F, Cook HT, Lamb JR, Bugeon L, Dallman MJet al., 2016, Mucosal inflammation at the respiratory interface: a zebrafish model, AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, Vol: 310, Pages: L551-L561, ISSN: 1040-0605

JOURNAL ARTICLE

Broncel M, Serwa RA, Ciepla P, Krause E, Dallman MJ, Magee AI, Tate EWet al., 2015, Multifunctional Reagents for Quantitative Proteome-Wide Analysis of Protein Modification in Human Cells and Dynamic Profiling of Protein Lipidation During Vertebrate Development, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, Vol: 54, Pages: 5948-5951, ISSN: 1433-7851

JOURNAL ARTICLE

Broncel M, Serwa RA, Ciepla P, Krause E, Dallman MJ, Magee AI, Tate EWet al., 2015, Myristoylation profiling in human cells and zebrafish., Data Brief, Vol: 4, Pages: 379-383, ISSN: 2352-3409

Human cells (HEK 293, HeLa, MCF-7) and zebrafish embryos were metabolically tagged with an alkynyl myristic acid probe, lysed with an SDS buffer and tagged proteomes ligated to multifunctional capture reagents via copper-catalyzed alkyne azide cycloaddition (CuAAC). This allowed for affinity enrichment and high-confidence identification, by delivering direct MS/MS evidence for the modification site, of 87 and 61 co-translationally myristoylated proteins in human cells and zebrafish, respectively. The data have been deposited to ProteomeXchange Consortium (Vizcaíno et al., 2014 Nat. Biotechnol., 32, 223-6) (PXD001863 and PXD001876) and are described in detail in Multifunctional reagents for quantitative proteome-wide analysis of protein modification in human cells and dynamic protein lipidation during vertebrate development׳ by Broncel et al., Angew. Chem. Int. Ed.

JOURNAL ARTICLE

Chen L, Alexandrov Y, Kumar S, Andrews N, Dallman MJ, French PMW, McGinty Jet al., 2015, Mesoscopic in vivo 3-D tracking of sparse cell populations using angular multiplexed optical projection tomography, BIOMEDICAL OPTICS EXPRESS, Vol: 6, Pages: 1253-1261, ISSN: 2156-7085

JOURNAL ARTICLE

Correia T, Lockwood N, Kumar S, Yin J, Ramel M-C, Andrews N, Katan M, Bugeon L, Dallman MJ, McGinty J, Frankel P, French PMW, Arridge Set al., 2015, y Accelerated Optical Projection Tomography Applied to In Vivo Imaging of Zebrafish, PLOS ONE, Vol: 10, ISSN: 1932-6203

JOURNAL ARTICLE

Jones PJM, Sim A, Taylor HB, Bugeon L, Dallman MJ, Pereira B, Stumpf MPH, Liepe Jet al., 2015, Inference of random walk models to describe leukocyte migration, PHYSICAL BIOLOGY, Vol: 12, ISSN: 1478-3967

JOURNAL ARTICLE

Chen L, Kumar S, Kelly D, Andrews N, Dallman MJ, French PMW, McGinty Jet al., 2014, Remote focal scanning optical projection tomography with an electrically tunable lens, BIOMEDICAL OPTICS EXPRESS, Vol: 5, Pages: 3367-3375, ISSN: 2156-7085

JOURNAL ARTICLE

Ciepla P, Konitsiotis AD, Serwa RA, Masumoto N, Leong WP, Dallman MJ, Magee AI, Tate EWet al., 2014, New chemical probes targeting cholesterylation of Sonic Hedgehog in human cells and zebrafish, CHEMICAL SCIENCE, Vol: 5, Pages: 4249-4259, ISSN: 2041-6520

JOURNAL ARTICLE

Progatzky F, Sangha NJ, Yoshida N, McBrien M, Cheung J, Shia A, Scott J, Marchesi JR, Lamb JR, Bugeon L, Dallman MJet al., 2014, Intestinal inflammation induced by dietary cholesterol in Zebrafish, 9th European-Mucosal-Immunology-Group Meeting, Publisher: WILEY-BLACKWELL, Pages: 27-28, ISSN: 0019-2805

CONFERENCE PAPER

Progatzky F, Sangha NJ, Yoshida N, McBrien M, Cheung J, Shia A, Scott J, Marchesi JR, Lamb JR, Bugeon L, Dallman MJet al., 2014, Dietary cholesterol directly induces acute inflammasome-dependent intestinal inflammation, NATURE COMMUNICATIONS, Vol: 5, ISSN: 2041-1723

JOURNAL ARTICLE

Progatzky F, Dallman MJ, Lo Celso C, 2013, From seeing to believing: labelling strategies for in vivo cell-tracking experiments, INTERFACE FOCUS, Vol: 3, ISSN: 2042-8898

JOURNAL ARTICLE

Taylor HB, Liepe J, Barthen C, Bugeon L, Huvet M, Kirk PDW, Brown SB, Lamb JR, Stumpf MPH, Dallman MJet al., 2013, P38 and JNK have opposing effects on persistence of in vivo leukocyte migration in zebrafish, IMMUNOLOGY AND CELL BIOLOGY, Vol: 91, Pages: 60-69, ISSN: 0818-9641

JOURNAL ARTICLE

Antkowiak M, Torres-Mapa ML, McGinty J, Chahine M, Bugeon L, Rose A, Finn A, Moleirinho S, Okuse K, Dallman M, French P, Harding SE, Reynolds P, Gunn-Moore F, Dholakia Ket al., 2012, Towards gene therapy based on femtosecond optical transfection, BIOPHOTONICS: PHOTONIC SOLUTIONS FOR BETTER HEALTH CARE III, Vol: 8427, ISSN: 0277-786X

JOURNAL ARTICLE

Chen L, McGinty J, Taylor HB, Bugeon L, Lamb JR, Dallman MJ, French PMWet al., 2012, Incorporation of an experimentally determined MTF for spatial frequency filtering and deconvolution during optical projection tomography reconstruction, OPTICS EXPRESS, Vol: 20, Pages: 7323-7337, ISSN: 1094-4087

JOURNAL ARTICLE

Gentle ME, Rose A, Bugeon L, Dallman MJet al., 2012, Noncanonical Notch Signaling Modulates Cytokine Responses of Dendritic Cells to Inflammatory Stimuli, JOURNAL OF IMMUNOLOGY, Vol: 189, Pages: 1274-1284, ISSN: 0022-1767

JOURNAL ARTICLE

Le Friec G, Shamoun S, Couzi L, Fremeaux-Bacchi V, Baker A, Bugeon L, Dallman M, Handford P, Lea S, Kemper Cet al., 2012, Jagged1 is a CD46 ligand and disturbance in CD46/Jagged 1 interaction leads to abnormal Th1 function and infections in humans, European Congress of Immunology, Publisher: WILEY-BLACKWELL, Pages: 8-8, ISSN: 0019-2805

CONFERENCE PAPER

Le Friec G, Sheppard D, Whiteman P, Karsten CM, Shamoun SA-T, Laing A, Bugeon L, Dallman MJ, Melchionna T, Chillakuri C, Smith RA, Drouet C, Couzi L, Fremeaux-Bacchi V, Koehl J, Waddington SN, McDonnell JM, Baker A, Handford PA, Lea SM, Kemper Cet al., 2012, The CD46-Jagged1 interaction is critical for human T(H)1 immunity, NATURE IMMUNOLOGY, Vol: 13, Pages: 1213-+, ISSN: 1529-2908

JOURNAL ARTICLE

Liepe J, Taylor H, Barnes CP, Huvet M, Bugeon L, Thorne T, Lamb JR, Dallman MJ, Stumpf MPHet al., 2012, Calibrating spatio-temporal models of leukocyte dynamics against in vivo live-imaging data using approximate Bayesian computation, INTEGRATIVE BIOLOGY, Vol: 4, Pages: 335-345, ISSN: 1757-9694

JOURNAL ARTICLE

Progatzky F, Taylor H, Bugeon L, Cassidy S, Radbruch A, Dallman MJ, Lamb JRet al., 2012, The role of Nfil3 in zebrafish hematopoiesis, DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, Vol: 38, Pages: 187-192, ISSN: 0145-305X

JOURNAL ARTICLE

Rose A, Kay E, Wren BW, Dallman MJet al., 2012, The Campylobacter jejuni NCTC11168 capsule prevents excessive cytokine production by dendritic cells, MEDICAL MICROBIOLOGY AND IMMUNOLOGY, Vol: 201, Pages: 137-144, ISSN: 0300-8584

JOURNAL ARTICLE

Bugeon L, Taylor HB, Progatzky F, Lin MI, Ellis CD, Welsh N, Smith E, Vargesson N, Gray C, Renshaw SA, Chico TJA, Zon LI, Lamb J, Dallman MJet al., 2011, The NOTCH pathway contributes to cell fate decision in myelopoiesis, HAEMATOLOGICA-THE HEMATOLOGY JOURNAL, Vol: 96, Pages: 1753-1760, ISSN: 0390-6078

JOURNAL ARTICLE

Kumar S, Alibhai D, Margineanu A, Laine R, Kennedy G, McGinty J, Warren S, Kelly D, Alexandrov Y, Munro I, Talbot C, Stuckey DW, Kimberly C, Viellerobe B, Lacombe F, Lam EW-F, Taylor H, Dallman MJ, Stamp G, Murray EJ, Stuhmeier F, Sardini A, Katan M, Elson DS, Neil MAA, Dunsby C, French PMWet al., 2011, FLIM FRET Technology for Drug Discovery: Automated Multiwell-Plate High-Content Analysis, Multiplexed Readouts and Application in Situ, CHEMPHYSCHEM, Vol: 12, Pages: 609-626, ISSN: 1439-4235

JOURNAL ARTICLE

Lara R, Mauri F, Taylor H, Derua R, Shia A, Gray C, Nicols A, Shiner RJ, Schofield EE, Bates P, Waelkens E, Dallman M, Lamb J, Zicha D, Downward J, Seckl M, Pardo Oet al., 2011, IDENTIFICATION OF RSK1 AS A KEY MODULATOR OF LUNG CANCER METASTASIS, JOURNAL OF THORACIC ONCOLOGY, Vol: 6, Pages: S514-S515, ISSN: 1556-0864

JOURNAL ARTICLE

Lara R, Mauri FA, Taylor H, Derua R, Shia A, Gray C, Nicols A, Shiner RJ, Schofield E, Bates PA, Waelkens E, Dallman M, Lamb J, Zicha D, Downward J, Seckl MJ, Pardo OEet al., 2011, An siRNA screen identifies RSK1 as a key modulator of lung cancer metastasis, ONCOGENE, Vol: 30, Pages: 3513-3521, ISSN: 0950-9232

JOURNAL ARTICLE

Lara R, Mauri FA, Taylor H, Derua R, Shia A, Gray C, Nicols A, Shiner RJ, Schofield E, Bates PA, Waelkens E, Dallman M, Lamb J, Zicha D, Downward J, Seckl MJ, Pardo OEet al., 2011, An siRNA screen identifies Rsk1 as a key modulator of lung cancer metastasis, CANCER RESEARCH, Vol: 71, ISSN: 0008-5472

JOURNAL ARTICLE

McGinty J, Taylor HB, Chen L, Bugeon L, Lamb JR, Dallman MJ, French PMWet al., 2011, In vivo fluorescence lifetime optical projection tomography, BIOMEDICAL OPTICS EXPRESS, Vol: 2, Pages: 1340-1350, ISSN: 2156-7085

JOURNAL ARTICLE

This data is extracted from the Web of Science and reproduced under a licence from Thomson Reuters. You may not copy or re-distribute this data in whole or in part without the written consent of the Science business of Thomson Reuters.

Request URL: http://wlsprd.imperial.ac.uk:80/respub/WEB-INF/jsp/search-html.jsp Request URI: /respub/WEB-INF/jsp/search-html.jsp Query String: respub-action=search.html&id=00153589&limit=30&person=true