Imperial College London

DrMarcoDi Antonio

Faculty of Natural SciencesDepartment of Chemistry

BBSRC Fellow
 
 
 
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Contact

 

+44 (0)20 7594 5866m.di-antonio

 
 
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Location

 

207LMolecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Weber:2019:10.1158/0008-5472.CAN-19-0691,
author = {Weber, J and Bollepalli, L and Belenguer, AM and Antonio, MD and De, Mitri N and Joseph, J and Balasubramanian, S and Hunter, CA and Bohndiek, SE},
doi = {10.1158/0008-5472.CAN-19-0691},
journal = {Cancer Res},
pages = {5407--5417},
title = {An Activatable Cancer-Targeted Hydrogen Peroxide Probe for Photoacoustic and Fluorescence Imaging.},
url = {http://dx.doi.org/10.1158/0008-5472.CAN-19-0691},
volume = {79},
year = {2019}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Reactive oxygen species play an important role in cancer, however, their promiscuous reactivity, low abundance, and short-lived nature limit our ability to study them in real time in living subjects with conventional noninvasive imaging methods. Photoacoustic imaging is an emerging modality for in vivo visualization of molecular processes with deep tissue penetration and high spatiotemporal resolution. Here, we describe the design and synthesis of a targeted, activatable probe for photoacoustic imaging, which is responsive to one of the major and abundant reactive oxygen species, hydrogen peroxide (H2O2). This bifunctional probe, which is also detectable with fluorescence imaging, is composed of a heptamethine carbocyanine dye scaffold for signal generation, a 2-deoxyglucose cancer localization moiety, and a boronic ester functionality that specifically detects and reacts to H2O2. The optical properties of the probe were characterized using absorption, fluorescence, and photoacoustic measurements; upon addition of pathophysiologic H2O2 concentrations, a clear increase in fluorescence and red-shift of the absorption and photoacoustic spectra were observed. Studies performed in vitro showed no significant toxicity and specific uptake of the probe into the cytosol in breast cancer cell lines. Importantly, intravenous injection of the probe led to targeted uptake and accumulation in solid tumors, which enabled noninvasive photoacoustic and fluorescence imaging of H2O2. In conclusion, the reported probe shows promise for the in vivo visualization of hydrogen peroxide. SIGNIFICANCE: This study presents the first activatable and cancer-targeted hydrogen peroxide probe for photoacoustic molecular imaging, paving the way for visualization of hydrogen peroxide at high spatiotemporal resolution in living subjects.Graphical Abstract: http://cancerres.aacrjournals.org/content/canres/79/20/5407/F1.large.jpg.
AU - Weber,J
AU - Bollepalli,L
AU - Belenguer,AM
AU - Antonio,MD
AU - De,Mitri N
AU - Joseph,J
AU - Balasubramanian,S
AU - Hunter,CA
AU - Bohndiek,SE
DO - 10.1158/0008-5472.CAN-19-0691
EP - 5417
PY - 2019///
SP - 5407
TI - An Activatable Cancer-Targeted Hydrogen Peroxide Probe for Photoacoustic and Fluorescence Imaging.
T2 - Cancer Res
UR - http://dx.doi.org/10.1158/0008-5472.CAN-19-0691
UR - https://www.ncbi.nlm.nih.gov/pubmed/31455691
VL - 79
ER -