Imperial College London
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DrMarcoDi Antonio

Faculty of Natural Sciences, Department of Chemistry

Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 5866m.di-antonio

 
 
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Location

 

207LMolecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Hansel-Hertsch:2016:10.1038/ng.3662,
author = {Hansel-Hertsch, R and Beraldi, D and Lensing, SV and Marsico, G and Zyner, K and Parry, A and Di, Antonio M and Pike, J and Kimura, H and Narita, M and Tannahill, D and Balasubramanian, S},
doi = {10.1038/ng.3662},
journal = {Nature Genetics},
pages = {1267--1272},
title = {G-quadruplex structures mark human regulatory chromatin},
url = {http://dx.doi.org/10.1038/ng.3662},
volume = {48},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - G-quadruplex (G4) structural motifs have been linked to transcription1,2, replication3 and genome instability4,5 and are implicated in cancer and other diseases6,7,8. However, it is crucial to demonstrate the bona fide formation of G4 structures within an endogenous chromatin context9,10. Herein we address this through the development of G4 ChIP–seq, an antibody-based G4 chromatin immunoprecipitation and high-throughput sequencing approach. We find ∼10,000 G4 structures in human chromatin, predominantly in regulatory, nucleosome-depleted regions. G4 structures are enriched in the promoters and 5′ UTRs of highly transcribed genes, particularly in genes related to cancer and in somatic copy number amplifications, such as MYC. Strikingly, de novo and enhanced G4 formation are associated with increased transcriptional activity, as shown by HDAC inhibitor–induced chromatin relaxation and observed in immortalized as compared to normal cellular states. Our findings show that regulatory, nucleosome-depleted chromatin and elevated transcription shape the endogenous human G4 DNA landscape.
AU  - Hansel-Hertsch,R
AU  - Beraldi,D
AU  - Lensing,SV
AU  - Marsico,G
AU  - Zyner,K
AU  - Parry,A
AU  - Di,Antonio M
AU  - Pike,J
AU  - Kimura,H
AU  - Narita,M
AU  - Tannahill,D
AU  - Balasubramanian,S
DO  - 10.1038/ng.3662
EP  - 1272
PY  - 2016///
SN  - 1061-4036
SP  - 1267
TI  - G-quadruplex structures mark human regulatory chromatin
T2  - Nature Genetics
UR  - http://dx.doi.org/10.1038/ng.3662
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000384391600023&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://www.nature.com/articles/ng.3662#Abs2
VL  - 48
ER  -
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