Imperial College London
Alternate

Emeritus ProfessorMustafaDjamgoz

Faculty of Natural Sciences, Department of Life Sciences

Emeritus Professor in Cancer Biology
 
 
 
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Contact

 

+44 (0)20 7594 5370m.djamgoz

 
 
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Location

 

Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Rizaner:2016:10.1007/s00249-016-1170-x,
author = {Rizaner, N and Onkal, R and Fraser, SP and Pristerá, A and Okuse, K and Djamgoz, MB},
doi = {10.1007/s00249-016-1170-x},
journal = {European Biophysics Journal},
pages = {735--748},
title = {Intracellular calcium oscillations in strongly metastatic human breast and prostate cancer cells: control by voltage-gated sodium channel activity.},
url = {http://dx.doi.org/10.1007/s00249-016-1170-x},
volume = {45},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The possible association of intracellular Ca(2+) with metastasis in human cancer cells is poorly understood. We have studied Ca(2+) signaling in human prostate and breast cancer cell lines of strongly versus weakly metastatic potential in a comparative approach. Intracellular free Ca(2+) was measured using a membrane-permeant fluorescent Ca(2+)-indicator dye (Fluo-4 AM) and confocal microscopy. Spontaneous Ca(2+) oscillations were observed in a proportion of strongly metastatic human prostate and breast cancer cells (PC-3M and MDA-MB-231, respectively). In contrast, no such oscillations were observed in weakly/non metastatic LNCaP and MCF-7 cells, although a rise in the resting Ca(2+) level could be induced by applying a high-K(+) solution. Various parameters of the oscillations depended on extracellular Ca(2+) and voltage-gated Na(+) channel activity. Treatment with either tetrodotoxin (a general blocker of voltage-gated Na(+) channels) or ranolazine (a blocker of the persistent component of the channel current) suppressed the Ca(2+) oscillations. It is concluded that the functional voltage-gated Na(+) channel expression in strongly metastatic cancer cells makes a significant contribution to generation of oscillatory intracellular Ca(2+) activity. Possible mechanisms and consequences of the Ca(2+) oscillations are discussed.
AU  - Rizaner,N
AU  - Onkal,R
AU  - Fraser,SP
AU  - Pristerá,A
AU  - Okuse,K
AU  - Djamgoz,MB
DO  - 10.1007/s00249-016-1170-x
EP  - 748
PY  - 2016///
SN  - 0175-7571
SP  - 735
TI  - Intracellular calcium oscillations in strongly metastatic human breast and prostate cancer cells: control by voltage-gated sodium channel activity.
T2  - European Biophysics Journal
UR  - http://dx.doi.org/10.1007/s00249-016-1170-x
VL  - 45
ER  -
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