Imperial College London

ProfessorMichaelJohnson

Faculty of MedicineDepartment of Brain Sciences

Professor of Neurology and Genomic Medicine
 
 
 
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Contact

 

m.johnson Website

 
 
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Location

 

E419Burlington DanesHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Roncon:2015:10.1038/srep14143,
author = {Roncon, P and Soukupova, M and Binaschi, A and Falcicchia, C and Zucchini, S and Ferracin, M and Langley, SR and Petretto, E and Johnson, MR and Marucci, G and Michelucci, R and Rubboli, G and Simonato, M},
doi = {10.1038/srep14143},
journal = {Scientific Reports},
title = {MicroRNA profiles in hippocampal granule cells and plasma of rats with pilocarpine-induced epilepsy - comparison with human epileptic samples},
url = {http://dx.doi.org/10.1038/srep14143},
volume = {5},
year = {2015}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - The identification of biomarkers of the transformation of normal to epileptic tissue would help to stratify patients at risk of epilepsy following brain injury, and inform new treatment strategies. MicroRNAs (miRNAs) are an attractive option in this direction. In this study, miRNA microarrays were performed on laser-microdissected hippocampal granule cell layer (GCL) and on plasma, at different time points in the development of pilocarpine-induced epilepsy in the rat: latency, first spontaneous seizure and chronic epileptic phase. Sixty-three miRNAs were differentially expressed in the GCL when considering all time points. Three main clusters were identified that separated the control and chronic phase groups from the latency group and from the first spontaneous seizure group. MiRNAs from rats in the chronic phase were compared to those obtained from the laser-microdissected GCL of epileptic patients, identifying several miRNAs (miR-21-5p, miR-23a-5p, miR-146a-5p and miR-181c-5p) that were up-regulated in both human and rat epileptic tissue. Analysis of plasma samples revealed different levels between control and pilocarpine-treated animals for 27 miRNAs. Two main clusters were identified that segregated controls from all other groups. Those miRNAs that are altered in plasma before the first spontaneous seizure, like miR-9a-3p, may be proposed as putative biomarkers of epileptogenesis.
AU - Roncon,P
AU - Soukupova,M
AU - Binaschi,A
AU - Falcicchia,C
AU - Zucchini,S
AU - Ferracin,M
AU - Langley,SR
AU - Petretto,E
AU - Johnson,MR
AU - Marucci,G
AU - Michelucci,R
AU - Rubboli,G
AU - Simonato,M
DO - 10.1038/srep14143
PY - 2015///
SN - 2045-2322
TI - MicroRNA profiles in hippocampal granule cells and plasma of rats with pilocarpine-induced epilepsy - comparison with human epileptic samples
T2 - Scientific Reports
UR - http://dx.doi.org/10.1038/srep14143
UR - http://hdl.handle.net/10044/1/41255
VL - 5
ER -