Imperial College London


Faculty of EngineeringDepartment of Electrical and Electronic Engineering

Research Associate



+44 (0)20 7594 0742m.kalofonou Website




B422 - Centre for Bio-Inspired Technology (CBIT)Bessemer BuildingSouth Kensington Campus





Melpomeni Kalofonou received the Dipl.-Eng. degree in Electrical and Computer Engineering in 2007 from the University of Patras, Greece followed by an MSc in Biomedical Engineering in the Department of Bioengineering in 2009 and a PhD from the Department of Electrical and Electronic Engineering in 2013 as part of the Centre for Bio-Inspired Technology, Imperial College London.

Her PhD research focused on the application of semiconductor technology in detection of cancer biomarkers using microchip based chemical sensors (Ion-Sensitive Field-Effect Transistors, ISFETs) integrated with current-mode circuits as part of Lab-on-Chip diagnostic platforms. Her work led to the demonstration of the first proof-of-concept method using ISFETs for real-time amplification and detection of DNA methylation, an epigenetic biomarker contributing significantly in the development and progression of cancer.

She continued on to become a Postdoctoral Researcher and lead the Cancer Engineering Technology initiative within the Centre for Bio-Inspired Technology. Her current research focuses on the use of fully integrated microchip technology as a diagnostic platform for detection of breast cancer minimal residual disease and relapse as well as monitoring of resistance to treatment, targeting tumour specific cell-free DNA markers through pH-sensitive isothermal DNA amplification chemistries. Her research interests also expand in the development of microchip sensing arrays for detection and quantification of DNA methylation as well as algorithms for feature extraction, classification and prediction based on real-time sensor data. 

Dr Kalofonou is a member of the IEEE, IET, ESMO and has been elected as a member of the Biomedical Circuits and Systems Technical Committee (BioCAS TC).

Project Websites:

Genetic Technology

Cancer Technology



Kottorou A, Antonacopoulou A, Dimitrakopoulos F, et al., Deregulation of methylation of transcribed-ultra conserved regions in colorectal cancer and their diagnostic and prognostic value, Journal of Clinical Oncology - Asco 2017

Sakellakis M, Koutras A, Pittaka M, et al., 2016, Identification of a seasonal pattern to brain metastases, Neuropsychiatric Disease and Treatment, Vol:12, ISSN:1178-2021, Pages:609-610

Dimitrakopoulos F-ID, Antonacopoulou AG, Kottorou A, et al., 2015, Variant of BCL3 gene is strongly associated with five-year survival of non-small-cell lung cancer patients, Lung Cancer, Vol:89, ISSN:0169-5002, Pages:311-319


Moser N, Rodriguez-Manzano J, Yu L-S, et al., Live Demonstration: A CMOS-Based ISFET Array for Rapid Diagnosis of the Zika Virus, IEEE International Symposium on Circuits and Systems (ISCAS) 2017

Koutsos A, Kalofonou M, Sohbati M, et al., 2016, Epigenetic-IC: A fully integrated sensing platform for epigenetic reaction monitoring, IEEE International Symposium on Circuits and Systems (ISCAS), IEEE, Pages:325-328, ISSN:2379-447X

More Publications