Imperial College London
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Professor Maria Kyrgiou

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Chair in Gynaecologic Oncology
 
 
 
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Contact

 

+44 (0)20 7594 2177m.kyrgiou Website

 
 
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Location

 

Institute of Reproductive and Developmental BiologyHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Halliwell:2016:10.1038/srep29494,
author = {Halliwell, DE and Morais, CLM and Lima, KMG and Trevisan, J and Siggel-King, MRF and Craig, T and Ingham, J and Martin, DS and Heys, KA and Kyrgiou, M and Mitra, A and Paraskevaidis, E and Theophilou, G and Martin-Hirsch, PL and Cricenti, A and Luce, M and Weightman, P and Martin, FL},
doi = {10.1038/srep29494},
journal = {Scientific Reports},
title = {Imaging cervical cytology with scanning near-field optical microscopy (SNOM) coupled with an IR-FEL},
url = {http://dx.doi.org/10.1038/srep29494},
volume = {6},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Cervical cancer remains a major cause of morbidity and mortality among women, especially in the developing world. Increased synthesis of proteins, lipids and nucleic acids is a pre-condition for the rapid proliferation of cancer cells. We show that scanning near-field optical microscopy, in combination with an infrared free electron laser (SNOM-IR-FEL), is able to distinguish between normal and squamous low-grade and high-grade dyskaryosis, and between normal and mixed squamous/glandular pre-invasive and adenocarcinoma cervical lesions, at designated wavelengths associated with DNA, Amide I/II and lipids. These findings evidence the promise of the SNOM-IR-FEL technique in obtaining chemical information relevant to the detection of cervical cell abnormalities and cancer diagnosis at spatial resolutions below the diffraction limit (≥0.2 μm). We compare these results with analyses following attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy; although this latter approach has been demonstrated to detect underlying cervical atypia missed by conventional cytology, it is limited by a spatial resolution of ~3 μm to 30 μm due to the optical diffraction limit.
AU  - Halliwell,DE
AU  - Morais,CLM
AU  - Lima,KMG
AU  - Trevisan,J
AU  - Siggel-King,MRF
AU  - Craig,T
AU  - Ingham,J
AU  - Martin,DS
AU  - Heys,KA
AU  - Kyrgiou,M
AU  - Mitra,A
AU  - Paraskevaidis,E
AU  - Theophilou,G
AU  - Martin-Hirsch,PL
AU  - Cricenti,A
AU  - Luce,M
AU  - Weightman,P
AU  - Martin,FL
DO  - 10.1038/srep29494
PY  - 2016///
SN  - 2045-2322
TI  - Imaging cervical cytology with scanning near-field optical microscopy (SNOM) coupled with an IR-FEL
T2  - Scientific Reports
UR  - http://dx.doi.org/10.1038/srep29494
UR  - http://hdl.handle.net/10044/1/34474
VL  - 6
ER  -
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