Publications
192 results found
Bieniasz PD, Rethwilm A, Pitman R, et al., 1995, A comparative study of higher primate foamy viruses, including a new virus from a gorilla., Virology, Vol: 207, Pages: 217-228, ISSN: 0042-6822
Few foamy (spuma) retroviruses have been investigated in molecular detail, despite their previous isolation from several mamalian species, including ten neutralization serotypes from various primates. Here, we have studied a new gorilla foamy virus (SFV-Gg) and investigated its functional and phylogenetic relationship to the human (HFV) and other primate foamy viruses, including that recently described in orangutans (SFV-11). Nucleotide sequencing of PCR products obtained from the R/U5 region of the LTR, gag, and pol genes revealed a close relationship between HFV and three chimpanzee isolates (SFV-6, SFV-7, and SFV-cpz). The SFV-Gg, SFV-11, rhesus macaque (SFV-1), and African green monkey (SFV-3) isolates were more divergent. To explore functional relationships, primate foamy virus transactivation of HFV LTR driven beta-galactosidase expression in a newly constructed cell line, BHLL, was investigated. HFV, SFV-6, and SFV-7 potently transactivated HFV LTR driven lacZ gene expression, SFV-Gg induced expression approximately 10-fold less efficiently, and SFV types 1, 2, 3, and 11 did not significantly transactivate the HFV LTR. It was, thus, possible to assay serum neutralizing activity in SFV-infected primates against HFV, SFV-6, and SFV-7 by reduction of beta-galactosidase activity following infection of the indicator cell line. Sera from infected chimpanzees and gorillas neutralized, to varying degrees, each of these three viruses, whereas orangutan sera did not. Our results, based on DNA sequences and functional assays, support the conclusion that HFV is closely related to foamy viruses of chimpanzee origin.
SHATTUCKEIDENS D, MCCLURE M, SIMARD J, et al., 1995, A COLLABORATIVE SURVEY OF 80 MUTATIONS IN THE BRCA1 BREAST-CANCER AND OVARIAN-CANCER SUSCEPTIBILITY GENE - IMPLICATIONS FOR PRESYMPTOMATIC TESTING AND SCREENING, JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, Vol: 273, Pages: 535-541, ISSN: 0098-7484
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- Citations: 390
McClure MO, Bieniasz PD, Schulz TF, et al., 1994, Isolation of a new foamy retrovirus from orangutans., J Virol, Vol: 68, Pages: 7124-7130, ISSN: 0022-538X
We have isolated a new foamy virus from blood samples taken from two apparently healthy orangutans (Pongo pygmaeus). The older orangutan has since died with encephalopathy after a brief acute illness, while the younger one, his grandson, remains well. These animals and 12 other orangutans had specific antibodies to foamy virus as measured by immunofluorescence. The new foamy virus and the antisera showed strong and specific neutralization, with only weak cross-reaction with other simian foamy virus strains. Southern blotting with gag and env probes of human foamy virus and PCR amplification showed that the new foamy virus, designated SFV-11, is related to, yet distinct from, previously characterized strains from humans, chimpanzees, and monkeys.
Weber J, McClure M, 1993, HIV quiet but not silent., Nature, Vol: 364, ISSN: 0028-0836
WEBER J, MCCLURE M, 1993, HIV QUIET BUT NOT SILENT, NATURE, Vol: 364, Pages: 110-110, ISSN: 0028-0836
Vile RG, Ali M, Hunter E, et al., 1992, Identification of a generalised packaging sequence for D-type retroviruses and generation of a D-type retroviral vector., Virology, Vol: 189, Pages: 786-791, ISSN: 0042-6822
In order to construct vectors based upon D-type, rather than C-type, retroviruses, we have identified a 624-bp fragment of Mason-Pfizer monkey virus (MPMV) which constitutes a packaging sequence for at least two D-type retroviruses. When this fragment was included in an extensively deleted D-type vector genome, the D-type viruses MPMV and SRV-5, but not the C-type viruses MLV-A or MLV-E, rescued the vector RNA from HeLa cells. The recombinant virus stocks have the host range of the rescuing D-type virus as shown by expression of an internal (SV40-puromycin) cassette replacing the retroviral structural genes. The recombinant MPMV was specifically neutralized by anti-MPMV serum and receptor interference was demonstrated when it was plated on cells productively infected with wild type MPMV. When the putative D-type packaging sequence was removed from the vector genome, even though the other sequence elements required for efficient reverse transcription remained, the vector was no longer rescued from HeLa cells. These results complement the recent demonstration of broad specificity of rescue of a C-type vector (carrying only the packaging sequence of Mo-MLV) by several different C-type, but not D-type, viruses. Replacement of the D-type packaging sequence by most of the extended packaging sequence of Mo-MLV prevented the otherwise D-type vector from being rescued by D-type viruses and did not allow it to be rescued by C-type viruses. This was probably because of the incompatibility of the D-type vector sequences with the C-type retroviral proteins involved in viral reverse transcription and integration. Hence, we have localized a packaging sequence that is recognized by D-type, but not by C-type, retroviruses and have constructed a D-type vector which may be useful in gene transfer experiments.
McClure MO, Dalgleish AG, 1992, Human immunodeficiency virus and the immunology of infection., Baillieres Clin Obstet Gynaecol, Vol: 6, Pages: 1-12, ISSN: 0950-3552
MCCLURE MO, MOORE JP, BLANC DF, et al., 1992, INVESTIGATIONS INTO THE MECHANISM BY WHICH SULFATED POLYSACCHARIDES INHIBIT HIV-INFECTION INVITRO, AIDS RESEARCH AND HUMAN RETROVIRUSES, Vol: 8, Pages: 19-26, ISSN: 0889-2229
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- Citations: 166
MCCLURE MO, 1991, THE SIMIAN IMMUNODEFICIENCY VIRUSES, MOLECULAR ASPECTS OF MEDICINE, Vol: 12, Pages: 247-253, ISSN: 0098-2997
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- Citations: 2
McClure MO, Whitby D, Patience C, et al., 1991, Dextrin sulphate and fucoidin are potent inhibitors of HIV infection in vitro., Antiviral Chem. and Chemother., Vol: 2, Pages: 149-156
WEISS RA, CLAPHAM PR, MCCLURE MO, et al., 1988, HUMAN IMMUNODEFICIENCY VIRUSES - NEUTRALIZATION AND RECEPTORS, JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES AND HUMAN RETROVIROLOGY, Vol: 1, Pages: 536-541, ISSN: 1077-9450
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- Citations: 27
MCCLURE MO, SATTENTAU QJ, BEVERLEY PCL, et al., 1987, HIV-INFECTION OF PRIMATE LYMPHOCYTES AND CONSERVATION OF THE CD4 RECEPTOR, NATURE, Vol: 330, Pages: 487-489, ISSN: 0028-0836
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- Citations: 113
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